福建林业科技2025，Vol.52Issue(2)：66-69,79,5.DOI:10.13428/j.cnki.fjlk.2025.02.009
朱顶红组织培养技术
The Tissue Culture Technology of Hippeastrum vittatum
摘要
Abstract
Using the bulbs and scapes of the newly developed Hippeastrum cultivar'Xue Ran Chu Xia'as explants,the optimal dis-infection time for explants,and the best media for bud induction,proliferation,and rooting in tissue culture were investigated.The results showed that the optimal disinfection time with HgCl2was 20 minutes for both bulbs and scapes,with survival rates of 83.3%and 66.7%,respectively.The highest induction rate for bulbs reached 83.3%on MS medium supplemented with 2.0 mg·L-1 6-BA,while the induction rate for scapes was 46.7%on MS medium supplemented with 1.0 mg·L-1 6-BA.The optimal proliferation medium for adventitious buds was MS medium supplemented with 2.0 mg·L-1 6-BA,1.0 mg·L-1 KT,0.6 mg·L-1 NAA and 30 g·L-1 sucrose.The best rooting medium was 1/2 MS medium supplemented with 0.4 mg·L-1 IBA,15 g·L-1 sucrose,and 0.2 g·L-1 activated carbon,resulting in a rooting rate of 100%.关键词
朱顶红/组织培养/鳞茎/花柄Key words
Hippeastrum vittatum/tissue culture/bulb/flower stalks分类
农业科技引用本文复制引用
陈翔,陈春,郑静,林佳佳,吴鹏程,姬语潞..朱顶红组织培养技术[J].福建林业科技,2025,52(2):66-69,79,5.基金项目
福建省农业引导性项目(2023N0027) （2023N0027）
