福建农业科技2025，Vol.56Issue(5)：41-47,7.DOI:10.13651/j.cnki.fjnykj.2025.05.007
云南金花茶叶片愈伤组织诱导研究
Study on Callus Induction from Leaf Explants of Camellia fascicularis
摘要
Abstract
In order to screen the optimal medium formulation for callus induction from leaves of Camellia fascicularis,the leaves of tissue-cultured seedlings of Camellia fascicularis were used as the materials,the effects of hormones,light conditions and placement methods on the callus induction of leaves were discussed.The formula of callus induction medium was optimized through the orthogonal experiment.The results showed that the methods of contacting the blade back of the leaf with the medium and maintaining the complete darkness during the whole process of cultivation were most conducive to the callus induction.The optimal medium formulation for leaf callus induction was 1/2MS+45 g·L-1 sucrose+3.0 mg·L-1 NAA.This study established the technical system for callus induction from the leaves of Camellia fascicularis,which laid a preliminary foundation for the subsequent development of genetic transformation system by using callus.关键词
云南金花茶/愈伤组织/诱导/植物生长调节剂Key words
Camellia fascicularis/Callus/Induction/Plant growth regulators分类
农业科技引用本文复制引用
田自能,陈思慧,王何泽,唐军荣..云南金花茶叶片愈伤组织诱导研究[J].福建农业科技,2025,56(5):41-47,7.基金项目
云南省农业基础研究联合专项(202101BD070001-045) （202101BD070001-045）
国际植物园联盟项目(BGCI2063076). （BGCI2063076）
