渔业研究2025，Vol.47Issue(4)：395-407,13.DOI:10.14012/j.jfr.2024164
长汀吻虾虎鱼线粒体基因组及系统发育研究
Study on the mitochondrial genome and phylogeny of Rhinogobius changtinensis
摘要
Abstract
[Objective]The genus Rhinogobius is highly diverse in species,and exhibits significant variation in morphological traits,making traditional morphological methods unreliable for accurate species identification and classification.R.changtinensis,a species in this genus,lacks clear mitochondrial genomic data,and its phylogenetic relationships remain poorly understood.This study aims to analyze the mitochondrial genome of R.changtinensis and provide insights into species identification,classification,and the phylogenetic study of the genus Rhinogobius.[Methods]Using the Illumina NovaSeq X Plus platform,the study conducted next-genera-tion sequencing on the mitochondrial genome of R.changtinensis,followed by the assembly and annotation of the genome sequence,and then analyzed the structural features and base composition of the mitochondrial gen-ome.Based on the nucleotide sequences of 13 protein-coding genes,the study constructed a phylogenetic tree using maximum likelihood(ML)and Bayesian inference(BI)methods.[Results]The mitochondrial genome of R.changtinensis was 16 487 bp in length,comprising 13 protein-coding genes,22 tRNA genes,2 rRNA genes,and an AT-rich region of 854 bp.The gene arrangement was consistent with most species within the genus.Phylogenetic analysis indicated that R.changtinensis was the most closely related to R.duospilus(PP=1,BS=100％).[Significance]This study provides valuable mitochondrial genomic data for R.changtinensis and offers crucial insights into the phylogenetic relationships within the genus Rhinogobius,contributing to the un-derstanding of its systematics.关键词
吻虾虎鱼/线粒体基因组/基因结构/系统发育Key words
Rhinogobius/mitochondrial genome/gene structure/phylogeny分类
农业科技引用本文复制引用
曾圣,陈小红,钟珅,杨德援,孟原正,林泽阳,韦舒健,朱雨晨,许贻斌..长汀吻虾虎鱼线粒体基因组及系统发育研究[J].渔业研究,2025,47(4):395-407,13.基金项目
福建省属公益类科研院所基本科研专项(2022R1013001) （2022R1013001）
福建省海洋服务与渔业高质量发展专项资金项目(FJHY-YYKJ-2024-1-18-4) （FJHY-YYKJ-2024-1-18-4）
