渔业研究2025，Vol.47Issue(4)：421-430,10.DOI:10.14012/j.jfr.2025034
栉江珧HSP70基因特征及其对温度胁迫的响应
Characteristics of HSP70 gene in Atrina pectinata and its response to temperature stress
摘要
Abstract
[Objective]Atrina pectinata is an important aquatic economic shellfish.Temperature is one of the key environmental factors affecting their growth,development and survival.This study aims to explore the phy-siological role of heat shock protein 70(HSP70)in A.pectinata response to temperature stress.[Methods]The complete domain fragments of HSP70 gene were obtained from the A.pectinata transcriptome library.The cDNA sequence was characterized by using bioinformatics analysis software.Tissue distribution and expres-sion levels of the HSP70 gene under different water temperatures(30,20,10 ℃)at different time points were analyzed using quantitative real-time polymerase chain reaction(qRT-PCR).[Results]The results showed that the open reading frame(ORF)of the HSP70 cDNA was 1 968 bp,which encoded 655 amino acids.The theoret-ical protein molecular weight was 71.51 kDa and the theoretical isoelectric point was 5.36.Multiple sequence alignment results showed that HSP70 in Chlamys farreri,Haliotis discus hannai,Mytilus galloprovincialis,Cyc-lina sinensis,Danio rerio,Mus musculus,and Homo sapiens had more than 85％homology.qRT-PCR results demonstrated ubiquitous expression of HSP70 across all examined tissues(foot,gill,adductor muscle,mantle,hepatopancreas,and gonads),with the foot exhibiting significantly higher relative expression levels compared to other tissues(P＜0.05).Under the condition of 30 ℃(high temperature),the relative expression level of HSP70 gene in gills showed a gradually decreasing trend at different time points.The relative expression level at 6 h was significantly higher than that at the other time points(P＜0.05),and the lowest was at 5 d.At 6 h,1 d and 3 d,the relative expression levels of HSP70 gene at 30 ℃ were all significantly higher than those at 20 ℃(control)(P＜0.05),while there was no significant difference in the relative expression level at 5 d compared with 20 ℃(control)(P＞0.05).Under the condition of 10 ℃(low temperature),the relative expression level of HSP70 gene in gills was the highest at 6 h,significantly higher than that at the other time points(P＜0.05);at 6 h and 10℃,the relative expression level of HSP70 gene was significantly higher than that at 20 ℃(control)(P＜0.05).[Conclusion]The study analyzes the structural features and expression patterns of HSP70 gene sequences in A.pectinata.It is speculated that HSP70 gene exists widely in the body and performs the most basic physiological functions in cells,and participates in the temperature stress response of A.pectinata.The results of this study provide a molecular theoretical basis for exploring the role of HSP70 gene in A.pectinata temperature stress.关键词
栉江珧/HSP70/序列分析/温度胁迫/表达分析Key words
Atrina pectinata/HSP70/sequence analysis/temperature stress/expression analysis分类
农业科技引用本文复制引用
李旋,何金霞,陈钰臻,刘朝阳,王腾腾,韩慧宗,王斐,李斌,刘相全..栉江珧HSP70基因特征及其对温度胁迫的响应[J].渔业研究,2025,47(4):421-430,10.基金项目
山东省重点研发计划项目(2021LZGC028) （2021LZGC028）
烟台市科技创新发展计划(2022XCZX089) （2022XCZX089）
