渔业研究2025，Vol.47Issue(4)：431-450,20.DOI:10.14012/j.jfr.2025043
鲍鱼内脏黄嘌呤氧化酶抑制肽的筛选与工艺优化
Screening and process optimization of xanthine oxidase inhibitory peptides from abalone viscera
摘要
Abstract
[Background]The Pacific abalone(Haliotis discus hannai)is one of the predominant aquaculture species in Fujian Province,China.During processing,visceral by-products-accounting for 15％-25％of live weight-are typically discarded,leading to significant resource wastage and environmental concerns.The ex-traction of bioactive peptides from these underutilized by-products represents a critical pathway for advancing value-added processing industries while addressing sustainability challenges.This approach not only mitigates resource inefficiency but also aligns with escalating demands for health-oriented functional products.Notably,the derived peptides exhibit antioxidant,anti-inflammatory,and antitumor properties,which have garnered significant attention for their potential applications in functional food development and pharmaceutical innova-tion.[Objective]To address the demand for high-value utilization of abalone visceral processing by-products,this study focuses on xanthine oxidase(XOD),a critical therapeutic target in hyperuricemia management,to es-tablish an XOD-inhibitory peptide screening system.By optimizing enzymatic hydrolysis conditions for aba-lone viscera,the research refines the bioactive peptide screening framework for abalone by-products,elucidates the relationship between key processing parameters and product bioactivity,and provides foundational data to advance industrial-scale development.These efforts aim to bridge the gap between sustainable resource utiliza-tion and the production of functional ingredients with therapeutic applications.[Methods]A combined strategy integrating single-factor experiments and response surface methodology was employed to systematically invest-igate the influence of enzymatic hydrolysis parameters on bioactive peptide preparation from abalone viscera.Leveraging the high-efficiency proteolytic properties of alkaline protease,dual evaluation metrics—degree of hydrolysis and XOD inhibitory rate-were utilized for process assessment.A process optimization model was developed using Box-Behnken design,with parameter interactions and their correlations to bioactivity elucid-ated through three-dimensional response surfaces and contour plot analyses.This approach quantitatively delin-eated the synergistic effects of enzymatic variables on functional outcomes,providing a robust framework for scalable bioactive peptide production.[Results]XOD detection system development:substrate-enzyme kinetic experiments were conducted to establish stable reaction conditions,including a substrate concentration of 0.24 mmol·L-1,XOD concentration of 20 μg·mL-1,pH 7.5,reaction temperature of 25 ℃,and reaction duration of 30 min.Enzymatic hydrolysis process optimization:the optimal parameter combination was determined as a solid-to-liquid ratio of 1∶50,enzyme dosage of 4000 U·g-1,temperature of 61 ℃,pH 9,and hydrolysis time of 4 h.The optimized hydrolysate exhibited an XOD inhibition rate of 89.71％and a degree of hydrolysis of 20.34％.[Significance]The integrated system combining XOD activity assay and enzymatic hydrolysis pro-cess optimization achieves the first targeted preparation of XOD-inhibitory peptides from abalone viscera.This methodology advances the development of bioactive peptides from marine processing by-products and demon-strates significant practical value in promoting resource utilization of abalone-derived waste.The framework es-tablishes a novel approach for precision enzyme-driven bioactive compound extraction,bridging functional in-gredient discovery with sustainable valorization of underutilized marine biomass.关键词
皱纹盘鲍/XOD抑制肽/鲍鱼内脏/酶解/多肽Key words
Haliotis discus hannai/XOD inhibitory peptide/abalone viscera/enzymatic hydrolysis/poylypeptide分类
农业科技引用本文复制引用
黄琼眉,乔琨,刘智禹,解辰阳,洪琳姗,黄文美,陈贝,许旻,蔡水淋,陈晓婷,林河通..鲍鱼内脏黄嘌呤氧化酶抑制肽的筛选与工艺优化[J].渔业研究,2025,47(4):431-450,20.基金项目
福建省海洋服务与渔业高质量发展专项(FJHY-YYKJ-2024-1-3) （FJHY-YYKJ-2024-1-3）
海洋渔业综合服务专项(FYZF-KTYJ-2025-3) （FYZF-KTYJ-2025-3）
福建省属公益类科研院所基本科研专项(2024R1047) （2024R1047）
福建省鲍鱼种业创新与产业化开发(2021FJSCZY02) （2021FJSCZY02）
