渔业研究2025，Vol.47Issue(4)：489-499,11.DOI:10.14012/j.jfr.2024136
Cu2+对香港牡蛎急性毒性和抗氧化酶活性的影响
Acute toxicity and antioxidant enzyme activity effects of Cu2+on Crassostrea hongkongensis
摘要
Abstract
[Background]Crassostrea hongkongensis is an economically valuable shellfish known for its deli-cious meat and rich nutritional content.Since the 21st century,with the increasing emission of heavy metals,the survival of C.hongkongensis has been greatly threatened.[Objective]The aim of this study is to explore the effect of Cu2+on the survival of C.hongkongensis.[Methods]Five treatment groups with copper concentra-tions of 40.00,56.62,79.98,113.24 and 160.00 mg/L were set up to carry out acute toxicity tests within 96 h,and to investigate the effects of the activities of catalase,total superoxide dismutase,glutathione peroxidase and glutathione-S transferase in different tissues(mantle,gill,hepatopancreas and gonad)of C.hongkongensis un-der Cu2+stress.[Results]Under Cu2+stress,the median lethal concentrations(LC50)of C.hongkongensis at 48,72 and 96 h were 116.01,84.33 and 65.32 mg/L,respectively,and the safe concentration(SC)was 0.65 mg/L.After 96 hours of Cu2+stress,the antioxidant indices in different tissues of C.hongkongensis were significantly affected(P＜0.05)with the extension of the experimental period.Gill tissue analysis revealed distinct enzymatic response patterns:CAT and T-SOD activities peaked before declining,GSH-Px fluctuated irregularly,and GST(or GSH-ST)followed a triphasic trend-initial decrease,transient increase,and subsequent reduction.In the mantle tissue,there was an upward trend observed for CAT,while T-SOD exhibited a decrease followed by an increase.GSH-Px and GSH-ST,on the other hand,displayed an initial increase before declining.In the hepato-pancreas,CAT demonstrated an ascending trend,with T-SOD showing a complex pattern of decrease,increase,and subsequent decrease.GSH-Px and GSH-ST initially increased before trending downwards.Within the gon-ads,both CAT and GSH-Px followed a pattern of initial increase followed by a decrease,T-SOD showed a de-crease-increase-decrease sequence,and GSH-ST decreased initially before increasing.[Conclusion]CAT in the hepatopancreas,T-SOD and GSH-ST in the gill,and GSH-Px in the gonad of C.hongkongensis were sensit-ive to Cu2+.This finding can be used as an important basis for the ecological monitoring of the concentration of Cu2+.The results of this experiment can provide reference for the healthy breeding of C.hongkongensis.关键词
香港牡蛎/铜离子(Cu2+)/急性毒性/抗氧化酶Key words
Crassostrea hongkongensis/copper ion(Cu2+)/acute toxicity/antioxidant enzyme分类
农业科技引用本文复制引用
蔡思灿,吴汉亮,张方琪,黄东明,栗志民..Cu2+对香港牡蛎急性毒性和抗氧化酶活性的影响[J].渔业研究,2025,47(4):489-499,11.基金项目
广东省科技厅项目(KTP20210288、KTP20240564) （KTP20210288、KTP20240564）
