分析化学2025，Vol.53Issue(7)：1097-1107,11.DOI:10.19756/j.issn.0253-3820.251022
基于铈-L-胱氨酸纳米酶比色检测对乙酰氨基酚
Colorimetric Detection of Acetaminophen Based on Cerium-L-Cysteine Nanozyme
摘要
Abstract
Nanomaterials(Ce-CS)with oxidase-like properties were synthesized in one step using L-cystine(CS)and ammonium cerium nitrate(CAN)as raw materials for detection of acetaminophen(APAP).The morphology,structure and elements composition of Ce-CS were characterized by scanning electron microscopy(SEM),energy dispersive X-ray spectroscopy(EDS),nitrogen adsorption specific surface area analysis(BET),X-ray diffraction(XRD),infrared spectroscopy(IR)and X-ray photoelectron spectroscopy(XPS).The Ce-CS with peroxidase-like activity could catalyze the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine(TMB)into blue oxided TMB(oxTMB),which had a significant absorption peak at 652 nm.Under the optimal catalytic conditions,i.e.,reaction temperature of 25℃(room temperature),pH=4.0,Ce-CS concentration of 1 mg/mL,and reaction time of 10 min,the catalytic mechanism and kinetics of Ce-CS were studied.When APAP existed in the reaction system,it could inhibit the peroxidase-like activity of Ce-CS,reduced the absorbance at 652 nm(A652),and the absorbance difference at 652 nm(ΔA652)had a good linear relationship with concentration of APAP in the range of 50-2000 μmol/L(R2=0.996),with a detection limit(S/N=3)of 0.1 μmol/L.This method was applied to detection of APAP in oral liquid and river water samples,with recoveries of 98.0%-102.0%,demonstrating the potential of Ce-CS as an oxidase substitute in drug analysis and environmental monitoring.关键词
铈-L-胱氨酸/类氧化酶/合成/比色法/对乙酰氨基酚Key words
Cerium-L-cysteine/Oxidase-like enzyme/Synthesis/Colorimetry/Acetaminophen引用本文复制引用
王艳艳,郑兰兰,曹红,邹洋洋,常军民..基于铈-L-胱氨酸纳米酶比色检测对乙酰氨基酚[J].分析化学,2025,53(7):1097-1107,11.基金项目
国家自然科学基金项目(No.21266029)和浙江省自然科学基金项目(No.LY17B060011)资助. Supported by the National Natural Science Foundation of China(No.21266029)and the Natural Science Foundation of Zhejiang Province,China(No.LY17B060011). （No.21266029）
