广东农业科学2025，Vol.52Issue(5)：76-86,11.DOI:10.16768/j.issn.1004-874X.2025.05.007
荔枝粗胫翠尺蛾卵黄原蛋白基因及其受体基因的克隆与生物信息学分析
Cloning and Bioinformatics Analysis of Vitellogenin Genes and Its Receptor Genes in Thalassodes immissaria
摘要
Abstract
[Objective]Vitellogenin(Vg)and its receptor(VgR)play key roles in insect reproduction by mediating nutrient transport during oocyte vitellogenesis.This study aimed to clone Vg and VgR from the Thalassodes immissaria,a notorious defoliator in lichee,and analyzed their bioinformatics characteristics and expression patterns across developmental stages to infer their roles in reproductive regulation.[Method]Using transcriptome data of T.immissaria,the full-length cDNA sequences of TiVg and TiVgR were cloned via PCR.Bioinformatics tools,such as ORF Finder,SignalP 5.0,ProtParam,SMART,TMHMM 2.0,and MEGA v5.05,were used for sequence analysis.Expression profiles of TiVg and TiVgR across different developmental stages and different tissues were quantified using transcriptomic TPM values and qRT-PCR.[Result]The open reading frames(ORFs)of TiVg and TiVgR span 5 343 and 5 250 bp respectively,encoding polypeptides of 1 780 and 1 749 amino acids,with predicted molecular weights of 202.403 and 196.248 kDa.Both TiVg and TiVgR are water-soluble,exhibiting hydropathicity index of-0.681 and-0.329,respectively.The N-termini of TiVg and TiVgR contain signal peptide regions composed of 16 and 14 amino acid residues,respectively.The phylogenetic analysis suggested that TiVg clusters within the Operophtera brumata ObVg clade,while TiVgR aligns closely with Helicoverpa armigera HaVgR orthologs.The expression pattern results with TPM value showed that TiVg expression initiated at the pupal stage and peaked in adult females,whereas TiVgR exhibited maximal expression in adult females with minimal levels detected in eggs.Tissue expression pattern suggested that TiVg was highly expressed in fat body of adult females and TiVgR was highly expressed in ovary of adult females,respectively.[Conclusion]The study successfully cloned Vg and VgR from T.immissaria.The conserved motifs and the expression patterns in both developmental stage and tissues suggest their potential roles in reproductive regulation.These findings provide a foundation for understanding reproductive mechanisms in T.immissaria.关键词
荔枝粗胫翠尺蛾/卵黄原蛋白/卵黄原蛋白受体/生物信息学分析/表达模式Key words
Thalassodes immissaria/vitellogenin/vitellogenin receptor/bioinformatics analysis/expression patterns分类
农业科技引用本文复制引用
朱国林,姚琼,李文景,徐淑,刘凯,段双刚..荔枝粗胫翠尺蛾卵黄原蛋白基因及其受体基因的克隆与生物信息学分析[J].广东农业科学,2025,52(5):76-86,11.基金项目
国家自然科学基金(32472564) （32472564）
国家荔枝龙眼产业技术体系专项(CARS-32) （CARS-32）
广东省农业和乡村振兴人才项目(NYQN202417) （NYQN202417）
广东省普通高校特色创新类项目(2023KTSCX046) （2023KTSCX046）
广东省农业科学院科技创新战略专项资金(高水平农科院建设)(R2024YJ-YB2001) （高水平农科院建设）
