广东农业科学2025，Vol.52Issue(5)：87-95,9.DOI:10.16768/j.issn.1004-874X.2025.05.008
浙江金线莲茎腐病病原菌鉴定及防治药剂室内毒力测定
Pathogen Identification of Stem Rot of Anoectochilus roxburghii and Laboratory Toxicity Test of Fungicides
摘要
Abstract
[Objective]The objective of this study is to clarify the pathogenic fungi and its taxonomic status that cause stem rot in Anoectochilus roxburghii under facility cultivation and aseptic cultivation modes,and to screen out the fungicides with better control effect on the disease by combining fungicide bioassay.[Method]The pathogenic fungi of A.roxburghii stem rot were obtained by tissue isolation,and the maximum likelihood method(ML)and Bayesian method were used for combined gene phylogenetic analysis,and the taxonomic status of pathogens was clarified by morphological identification.The mycelial growth rate method was used to screen out the fungicides with good effect on the pathogens of stem rot(ZJUP0240R and ZJUP1054-1).[Result]The results showed that the A.roxburghii stem rot was caused by Fusarium oxysporum(Fo)in both facility cultivation and aseptic cultivation modes.The results of fungicides bioassays showed that four fungicides,including tebuconazole,difenoconazole,epoxiconazole,and pyraclostrobin,exhibited good inhibitory effects against the pathogens Fo ZJUP0240R and Fo ZJUP1054-1 of A.roxburghii stem rot.Among them,tebuconazole had an EC50 value of 0.0652 mg/L and 0.0974 mg/L against Fo ZJUP0240R and Fo ZJUP1054-1,respectively;difenoconazole had an EC50 value 0.0731 mg/L and 0.1222 mg/L against Fo ZJUP0240R and Fo ZJUP1054-1,respectively;epoxiconazole had an EC50 value of 0.0166 mg/L and 0.0564 mg/L against Fo ZJUP0240R and Fo ZJUP1054-1 respectively;and pyraclostrobin had an EC50 value of 2.5657 mg/L and 0.3696 mg/L against Fo ZJUP0240R and Fo ZJUP1054-1,respectively.The toxic effects of the pathogens of A.roxburghii stem rot(F.oxysporum ZJUP0240R and F.oxysporum ZJUP1054-1)to the four tested fungicides were as follows:epoxiconazole>tebuconazole>difenoconazole>pyraclostrobin.[Conclusion]F.oxysporum is a pathogenic fungi of A.roxburghii stem rot under different cultivation modes,and the results of fungicide screening experiments show that triazole fungicides and strobilurins fungicides have a good effect on the mycelial inhibition of A.roxburghii stem rot pathogens,and the results of this study can provide a reference for the identification and chemical control of A.roxburghii stem rot.关键词
金线莲茎腐病/病原鉴定/尖孢镰刀菌/三唑类杀菌剂/甲氧基丙烯酸酯类杀菌剂/病害防控Key words
Anoectochilus roxburghii stem rot/pathogen identification/Fusarium oxysporum/triazole fungicides/strobilurins fungicides/disease control分类
农业科技引用本文复制引用
彭一文,钟朱佩琦,金颖,严晓妮,王国荣,吴梅,吴慧明,冯晓晓..浙江金线莲茎腐病病原菌鉴定及防治药剂室内毒力测定[J].广东农业科学,2025,52(5):87-95,9.基金项目
浙江省教育厅一般科研项目(Y202249712) （Y202249712）
金华市农业重大科技项目(2023-2-005) （2023-2-005）
