高等学校化学学报2025，Vol.46Issue(8)：17-24,8.DOI:10.7503/cjcu20250101
电导调控的双极电极电化学发光法检测尿素
Detection of Urea by Conductivity-regulated Bipolar Electrochemiluminescence
摘要
Abstract
In this study,a bipolar electrochemiluminescence(BPE-ECL)sensing platform based on the regulation of conductivity was constructed for the detection of urea.An independent sensing cell and a reporting cell were built on the patterned BPE,and ruthenium bipyridine/tri-n-propylamine was used as the source of ECL.When urea at different concentrations is present in the sensing cell,urease specifically catalyzes the decomposition of urea,generating ions that promote an increase in the conductivity.Based on the charge balance of BPE,the ECL intensity from the reporting cell increases.Meanwhile,the ECL intensities increase following the enhancement of urea concentrations.Therefore,a BPE-ECL platform based on the regulation of conductivity was established for the detection of urea.Under optimal conditions,this sensing platform exhibits a good linear relationship for urea in the range of 10 nmol/L to 1 mmol/L,and the limit of detection is 0.19 nmol/L(S/N=3).The sensing platform was further applied to spiked recovery detection in human urine samples,showing good accuracy and anti-interference ability.This sensing strategy not only eliminates the need for exogenous electroactive indicators,but also effectively avoids the interference of complex biological samples with the signal reaction through the sensing-signal separation of BPE.关键词
双极电极/电化学发光/电导调控/传感-信号分离/尿素Key words
Bipolar electrode(BPE)/Electrochemiluminescence(ECL)/Conductivity regulation/Sensing-signal separation/Urea分类
化学化工引用本文复制引用
杨碧芳,郭晓戈,戴小辉,杨伟强,倪建聪..电导调控的双极电极电化学发光法检测尿素[J].高等学校化学学报,2025,46(8):17-24,8.基金项目
福建省市场监督管理局科技项目(批准号:FJMS2023065)资助.Supported by the Science and Technology Project of Fujian Provincial Administration of Market Supervision,China(No.FJMS2023065). （批准号:FJMS2023065）
