影像科学与光化学2025，Vol.43Issue(5)：5-13,22,10.DOI:10.7517/issn.1674-0475.2025.05.01
谷胱甘肽响应型竹红菌类光敏剂用于光动力治疗
GSH-responsive Photosensitizer Based on Hypocrellin B for Photodynamic Therapy
摘要
Abstract
Objective:We designed and synthesized a glutathione(GSH)-responsive hypocrellin B(HB)derivative photosensitizer HB-DNB to explore the effect of GSH-responsive photodynamic therapy(PDT).Methods:HB-DNB was obtained by reacting HB with 2,4-dinitrobenzenesulfonyl chloride.The reactive performance of HB-DNB to GSH was investigated in solutions and cells,respectively.Results:HB-DNB was successfully synthesized.Fluorescence tests showed that GSH could significantly reduce the fluorescence intensity of HB-DNB at 668 nm,and it reached equilibrium when 40 μM of GSH was added for 100 minutes.The results of singlet oxygen(1O2)tests indicated that the ability of HB to generate 1O2 was restored after the addition of GSH.MTT assays demonstrated that HB-DNB had low dark toxicity,and treating with 4 μM HB-DNB could reduce the survival rates of both HeLa cells and A549 cells to less than 10%using 532 nm laser irradiation.Conclusion:The photosensitizer HB-DNB can not only react with GSH in solutions but also be selectively activated by GSH in tumor cells,restoring the characteristic emission wavelength and photodynamic effect of HB.This study provides new ideas for the application of natural photosensitizer and the exploration of enhancing the tumor targeting.关键词
竹红菌乙素/谷胱甘肽(GSH)/光动力治疗/肿瘤细胞/选择性激活Key words
hypocrellin B/glutathione(GSH)/photodynamic therapy/cancer cells/selective activation分类
医药卫生引用本文复制引用
于喆,刘田,刘卫敏,吴加胜,王颖,汪鹏飞..谷胱甘肽响应型竹红菌类光敏剂用于光动力治疗[J].影像科学与光化学,2025,43(5):5-13,22,10.基金项目
国家重点研发计划项目(2022YFA1207600). （2022YFA1207600）
