干旱地区农业研究2025，Vol.43Issue(4)：22-31,10.DOI:10.7606/j.issn.1000-7601.2025.04.03
不同桃种质资源1年生幼树抗寒性评价
Evaluation of cold resistance in one-year-old peach germplasm seedlings
摘要
Abstract
One-year-old seedlings of 21 peach germplasms,subjected to cold hardiness treatment at low tem-peratures,were used as experimental materials.To compare and analyze their cold tolerance,we calculated the le-thal temperature(LT50)and recorded the field survival rates.Meanwhile,physiological indicators were measured,and the relationship between survival rate and cold resistance was analyzed.Then,we used LT50 and subordinate function,respectively,to clarify the cold tolerance classification of twenty-one peach.Under cold stress,branches kept relatively high enzyme activity of phenylalanine ammonia-lyase(PAL).Moreover,the high content of malond-ialdehyde(MDA),free proline(Pro)and soluble sugar(SS)was detected,and LT50 was relatively lower(range from-26.65℃to-17.69℃).In the field experiment,the survival rate of the plants also decreased gradually with the decrease in temperature.When the temperature was below-20℃,varieties such as'Zhonghuashoutao'declined particularly significantly,with the survival rate all below 50%.When the temperature dropped to-25℃,the sur-vival rates of the varieties'Hangongzhu','Donghe 1',and'Hunchun'ranged between 80%and 90%,while those of the other varieties were all below 80%.When the temperature dropped to-30℃,the survival rate of all varieties fell to 0.By using the hierarchical cluster method,the cold resistance ability of 21 peach germplasm re-sources of young trees can be classified into five categories in sequence from extremely strong to weak:the first cat-egory was'Hangongzhu',the categories of 2,3,4,and 5 respectively contain 3,4,9,and 4 varieties.Correla-tion analysis showed that the survival rate was significantly positively with comprehensive evaluation value(D),PAL activity,contents of Pro,and SS,and significantly negatively correlated with the LT50 and MDA content.Sig-nificant differences in cold resistance were observed among young peach trees.Following low-temperature treatment,the PAL activity and the contents of MDA,Pro,and SS showed strong correlations with cold resistance,indicating they can serve as key indicators for assessing cold tolerance in young peach germplasm.关键词
桃/种质资源/早期抗寒性评价/半致死温度/生理指标Key words
peach/germplasm resources/early assessment of cold tolerance/LT50/physiological index分类
农业科技引用本文复制引用
李杰,田启航,王雨,刘国俭,王召元,田振东,常瑞丰,李永红..不同桃种质资源1年生幼树抗寒性评价[J].干旱地区农业研究,2025,43(4):22-31,10.基金项目
财政部和农业农村部:国家现代农业产业技术体系资助项目(CARS-30-Z-02) （CARS-30-Z-02）
河北省农林科学院科技创新专项课题(2022KJCXZX-CGS-6) （2022KJCXZX-CGS-6）
河北省现代农业产业技术体系创新团队建设项目(HBCT2023130205) （HBCT2023130205）
河北省热杂果现代种业科技创新团队项目(21326310D) （21326310D）
河北省农林科学院基本科研业务费青年基金(2023020102) （2023020102）
