干旱地区农业研究2025，Vol.43Issue(4)：43-52,10.DOI:10.7606/j.issn.1000-7601.2025.04.05
NO介导下藜麦LAZ1基因家族生信分析及盐碱胁迫下的表达模式
Bioinformatics analysis and expression patterns of the LAZ1 gene family in quinoa under saline-alkali stress mediated by nitric oxide
摘要
Abstract
The LAZ1 gene family members were identified from the quinoa genome using bioinformatics meth-ods,and their basic physicochemical properties,secondary and tertiary structures,phylogenetic relationships,chromosome localization,gene duplication,gene structure,and promoter cis-acting elements were analyzed.Tran-scriptome data coupled with RT-qPCR were used to analyze the expression patterns of LAZ1 family genes in differ-ent organs and times under mixed saline-alkali stress and exogenous NO treatment.The results showed that a total of 11 LAZ1 genes were identified in the quinoa genome,and the LAZ1 gene family was divided into 3 subfamilies by phylogenetic analysis.Fragment replication is the main reason for the evolution of this gene family,and it had un-dergone strong purification selection in the evolutionary process.On the basis of transcriptomic analysis,RT-qPCR analysis of 6 LAZ1 genes under salt-alkali stress and NO treatment showed that the expression of different LAZ1 genes in different tissues was responsive to mixed salt-alkali and positively regulated by NO,and the relative ex-pression of CqLAZ1-9 in roots under salt-alkali stress was increased by 129.6 times.In summary,this study identi-fied 11 members of the LAZ1 family from the quinoa genome,and different members showed different expression patterns in different tissues,among which CqLAZ1-9 has the strongest ability to saline-alkali stress and exogenous NO treatment.关键词
藜麦/LAZ1基因家族/NO/生信分析/盐碱胁迫Key words
quinoa/LAZ1 gene family/NO/bioinformatics analysis/saline-alkali stress分类
农业科技引用本文复制引用
贾子健,王宝强,桂金凤,朱晓林,陈立飞,魏小红,赵颖..NO介导下藜麦LAZ1基因家族生信分析及盐碱胁迫下的表达模式[J].干旱地区农业研究,2025,43(4):43-52,10.基金项目
国家自然科学基金(32301775) （32301775）
甘肃农业大学省部共建干旱生境作物学国家重点实验室开放基金课题(GSCS-2021-10) （GSCS-2021-10）
甘肃省自然科学基金(23JRRA1426) （23JRRA1426）
