果树学报2025，Vol.42Issue(7)：1442-1454,13.DOI:10.13925/j.cnki.gsxb.20240682
茉莉酸甲酯促进采后杧果成熟软化及其关键基因表达调控
Methyl jasmonate promotes postharvest ripening and softening of mango and regulates the expression of key genes
摘要
Abstract
[Objective]Mango is renowned as the"King of Tropical Fruits"for its delicious flesh and distinctive flavor.It is a respiratory climacteric fruit that turns yellow and soft after harvest due to a rap-id increase in respiratory rate.Ethephon(ETH)induces the generation of endogenous ethylene,thereby achieving the effect of rapidly initiating the ripening process.This process is non-adjustable and irre-versible,resulting in excessive over-ripening,softening,and rotting of a large number of fruits.It has become a key factor restricting the development of the mango industry and post-harvest storage and preservation.Therefore,there is an urgent need to study new ripening technologies to address the exist-ing issues and extend the shelf life of mangoes.A significant amount of research have indicated that ex-ogenous methyl jasmonate(MeJA)treatment plays a role in regulating the quality of post-harvest fruits.However,the mechanism of action of the MeJA on mangoes remains unclear.Therefore,this study ex-plored the influence and mechanism of the exogenous MeJA treatment on the quality of post-harvest mango fruits,providing theoretical support for the application of the MeJA in post-harvest storage and preservation of the fruits and offering new ideas to solve the problem of rapid rotting after ethylene treatment.[Methods]The test mango variety was Tainong No.1 harvested from the mango plantation in Baoping Village,Yazhou District,Sanya City,Hainan Province.Immediately after harvest,they were transported to the laboratory.The diseased and damaged fruits were screened and removed.The fruit stalks were cut off,and they were washed and soaked in 0.1％chlorinated detergent for 20 minutes to re-move residual pectin and pathogens.After drying,they were evenly divided into five groups.Different concentrations of the MeJA solutions of 0.5,1,and 2 mmol·L-1 were used for soaking treatment for 5 min-utes,followed by sealing for 20 hours.Ethephon treatment at 0.5 g·L-1(ETH)and water treatment were used as control groups.The chroma,hardness,ethylene release rate,and TSS physiological indicators of each group of the mangoes during storage were detected.Simultaneously,the effects of 1 mmol·L-1 Me-JA on the texture,protopectin content,soluble pectin content of mangoes were determined,and the spe-cific effects on the enzyme activities of key enzymes and the expression levels of key enzyme genes in the cell wall degradation pathway were analyzed in depth.[Results]Compared with the water control group,the 1 and 2 mmol·L-1 MeJA groups both promoted the degradation of chlorophyll,facilitated fruit color transformation,promoted the decrease in hardness,increased the ethylene release rate and soluble solids content,while the effect of the 0.5 mmol·L-1 group was not significant.Both the ETH group and 2 mmol·L-1 MeJA significantly promoted the rapid ripening of the fruits.The 1 mmol·L-1 treatment promoted the color transformation of mango fruits and inhibited the rate of hardness decrease compared with the ETH group.Additionally,compared with the water control group,the 1 mmol·L-1 MeJA could promote changes in the texture of the mangoes,facilitate the decrease in protopectin con-tent and the increase in soluble pectin content,increase the activities of polygalacturonase(PG),pectin methylesterase(PME),pectin lyase(PL),cellulase(CX),and the expression levels of their encoding genes MiPME,MiPL and MiPG.The correlation analysis was conducted on the hardness,texture,en-zyme activity,and gene expression levels of the Tainong No.1 mango fruits after the exogenous MeJA treatment.The PME enzyme activity was significantly and positively correlated with the MiPME2,the correlation between the PL enzyme activity and the MiPL2 gene expression was greater than those of the MiPLl gene,and MiPG enzyme activity was significantly and positively correlated with the MiPG3 gene expression.[Conclusion]The study showed that after the methyl jasmonate treatment,the differ-ential expression of cell wall degradation enzyme genes would lead to changes in the activities of PME,PL,and PG enzymes,promoting the transformation of protopectin to soluble pectin in the fruit,ulti-mately resulting in the softening of the mangoes.关键词
杧果/茉莉酸甲酯/贮藏保鲜/软化/基因表达Key words
Mango/Methyl jasmonate/Storage and preservation/Softening/Gene expression分类
农业科技引用本文复制引用
张莎莎,王鑫,郭盼,张竞,马德新,邵远志,李雯..茉莉酸甲酯促进采后杧果成熟软化及其关键基因表达调控[J].果树学报,2025,42(7):1442-1454,13.基金项目
海南农垦联合创新科研项目(HKKJ202432) （HKKJ202432）
海南大学芒果产业体系建设项目和国家重点研发计划项目(2023YFD2300803) （2023YFD2300803）
