医学分子生物学杂志2025，Vol.22Issue(4)：319-324,338,7.DOI:10.3870/j.issn.1672-8009.2025.04.003
Linc279227调控PINK1/Parkin介导的线粒体自噬影响糖尿病肾病小鼠肾损伤研究
Effect of Linc279227 on Renal Injury in Diabetes Nephropathy Mice via PINK1/Parkin Mediated Mitochondrial Autophagy
摘要
Abstract
Objective To explore the effect and mechanism of TONCS_00279227(Linc279227)on renal injury in diabetes nephropathy mice.Methods Mice were divided into a Control group and a Model group.After successful modeling of db/db mice in the Model group,the mice kidney tissues were taken.qRT-PCR was used to detect the expression levels of Linc279227,PINK1,and Parkin in mice kidney tissues.Western blotting was used to detect the protein expres-sion levels of LC3-Ⅱ,P62,PINK1,and Parkin in mice kidney tissues.Mouse renal podocyte MPC5 cells were divided into 3 groups:Vector group,Linc27922 group,and Linc27922 group.CCK8 and flow cytometry were used to detect cell proliferation and apoptosis.Western blotting was used to detect the expression levels of LC3-Ⅱ,P62,PINK1,and Parkin proteins in each group of cells.Results Compared with those in the Control group,the expression levels of Linc279227 and P62 protein in the renal tissues of the model group mice were significantly upregu-lated(P＜0.01),while the expression levels of PINK1 and Parkin mRNA and proteins and the LC3-Ⅱ protein were significantly downregulated(P＜0.01).Compared with those in the Vector group,the mRNA and protein expression levels of PINK1 and Parkin and the protein expression lev-el of LC3-Ⅱ in MPC5 cells of the Linc279227 group were significantly downregulated(P＜0.01),and the expression level of P62 was significantly upregulated(P＜0.01).Compared with those in the Linc279227 group,the LC3-Ⅱ expression level of MPC5 cells in the Linc27922+FCCP group was upregulated(P＜0.01),the expression level of P62 was significantly downregulated(P＜0.01),the cell proliferation ability was significantly increased(P＜0.05),and the cell apoptosis was signif-icantly reduced(P＜0.01).Conclusion Linc279227 can mediate mitochondrial autophagy of ne-phropodocytes by regulating the PINK1/Parkin pathway to affect renal injury in diabetes nephropathy mice.关键词
糖尿病肾病/Linc279227/自噬/PINK1/Parkin通路Key words
diabetes nephropathy/Linc279227/autophagy/PINK1/Parkin pathway分类
医药卫生引用本文复制引用
沈群,王利民,邓薇,熊文,谢红武..Linc279227调控PINK1/Parkin介导的线粒体自噬影响糖尿病肾病小鼠肾损伤研究[J].医学分子生物学杂志,2025,22(4):319-324,338,7.基金项目
湖南省自然科学基金(No.2024JJ7556) This work was supported by a grant from the Natural Science Foundation of Hunan Province(No.2024JJ7556) （No.2024JJ7556）
