医学分子生物学杂志2025，Vol.22Issue(4)：332-338,7.DOI:10.3870/j.issn.1672-8009.2025.04.005
c-Myc/miR-27b-3p上调Bcl-2促进多发性骨髓瘤存活的分子机制研究
c-Myc/miR-27b-3p Promote Multiple Myeloma Cell Survival by Upregulating Bcl-2
摘要
Abstract
Objective To explore the molecular mechanisms of c-Myc/miR-27b-3p/Bcl-2 axis on promoting multiple myeloma(MM)cell survival.Methods The relative expression levels of MM-related genes and microRNAs(miRNAs)were measured in human MM cell lines RPMI8226 and U266 cells by using qPCR analysis.TargetScan and dual-luciferase gene reporter assay were used to predict and verify the targeting relationship between miR-27b-3p and Bcl-2,respective-ly.The primary MM cells were isolated from 14 patients with MM recruited from the Fifth Affiliated Hospital of Xinjiang Medical University.The mRNA expression levels of c-Myc,Bcl-2,and the ex-pression level of miR-27b-3p in primary MM cells were detected by qPCR.Pearson correlation analy-sis was used to analyze the relationship among their expression levels.Adenovirus vectors with c-Myc or Bcl-2 overexpression(Bcl-2 OE,c-Myc OE),or miR-27b-3p inhibitor(miR-27b-3p inhibi-tor)were constructed and transfected into the BMSCs.The expression levels of p-STAT3,STAT3,p-JAK2,and JAK2 in BMSCs were detected by Western blotting.Results Compared with those in the BMSCs,the mRNA expression levels of c-Myc and Bcl-2 were upregulated in the RPMI8226 cells and the U266 cells(P＜0.01),and miR-27b-3p was downregulated in the RPMI8226 cells and the U266 cells(P＜0.01).Dual-luciferase gene reporter assay results showed that miR-27b-3p was directly targeted to the Bcl-2 3'-UTR.qPCR and Pearson correlation analysis showed that the relative expression levels of c-Myc mRNA and miR-27b-3p,miR-27b-3p and Bcl-2 mRNA were linearly and negatively correlated(P＜0.01),while the relative expression levels of c-Myc mRNA and Bcl-2 mRNA were linearly and positively correlated(P＜0.01).Compared with those in the BMSCs group,the expression levels of p-STAT3,STAT3,p-JAK2,and JAK2 in the BMSCs+c-Myc OE group,the BMSCs+miR-27b-3p inhibitor group,and the BMSCs+Bcl-2 OE group were all increased(P＜0.05).Conclusion c-Myc upregulation and miR-27b-3p inhibition activated the Bcl-2 to promote cell survival in MM.关键词
多发性骨髓瘤/c-Myc/miR-27b-3p/Bcl-2/JAK2/STAT3/细胞存活Key words
multiple myeloma/c-Myc/miR-27b-3p/Bcl-2/JAK2/STAT3/cell survival分类
医药卫生引用本文复制引用
薛琴,田新玮,王静,郑琦丽,艾比巴·阿不都拉..c-Myc/miR-27b-3p上调Bcl-2促进多发性骨髓瘤存活的分子机制研究[J].医学分子生物学杂志,2025,22(4):332-338,7.基金项目
新疆维吾尔自治区自然科学基金(No.2020D01C327) This work was supported by a grant from the Natural Science Foundation of Xinjiang Uygur Autonomous Region(No.2020D01C327) （No.2020D01C327）
