医学分子生物学杂志2025，Vol.22Issue(4)：339-345,7.DOI:10.3870/j.issn.1672-8009.2025.04.006
miR-200a-3p对新生大鼠缺氧缺血性脑损伤的作用和机制
Effect and Mechanism of miR-200a-3p on Hypoxic-ischemic Brain Damage in Neonatal Rats
摘要
Abstract
Objective To explore the effect of microRNA-200 a-3 p(miR-200a-3p)on hy-poxic-ischemic brain damage(HIBD)in neonatal rats.Methods Sixty neonatal SD rats were di-vided into 4 groups:sham operation group,HIBD group,miR-200a-3p antagomir group,and NC-antagomir group,15 cases in each group.The rat neonatal HIBD model was constructed.The expres-sion level of miR-200a-3p in the hippocampus was detected by quantitative PCR.The nerve function and brain water content were evaluated.The pathological changes and apoptosis of the hippocampal tissues were observed by HE staining and TUNEL staining.The levels of oxidative stress,inflamma-tory factors,and the expression levels of apoptosis-related proteins were detected by ELISA and Western blotting.Results Compared with those in the sham operation group,the expression level of miR-200a-3p in the hippocampus,the score of nerve function,the brain water content,the ap-optosis rate of brain cells,the levels of MDA,IL-6,IL-1β,TNF-α and the expression level of Cleaved-Caspase-3 were increased(P＜0.05),while the levels of SOD,CAT,Bcl-2/Bax,BD-NF and TrkB were decreased in the HIBD group(P＜0.05).Compared with those in the NC-an-tagomir group,the expression level of miR-200a-3p in the hippocampus,the score of nerve func-tion,the brain water content,the apoptosis rate of brain cells,the levels of MDA,IL-6,IL-1β,TNF-α and the expression level of Cleaved-Caspase-3 were decreased(P＜0.05),while the levels of SOD,CAT,Bcl-2/Bax,BDNF and TrkB were increased in the miR-200a-3p antagomir group(P＜0.05).Conclusion InhibitingmiR-200o-3pcan reduce nerve injury in neonatal rats with HIBD,which may be related to the inhibition of apoptosis and oxidative stress,and the regulation of BDNF/TrkB pathway.关键词
缺氧缺血性脑损伤/新生大鼠/微小RNA-200a-3pKey words
hypoxic ischemic brain damage/neonatal rat/microRNA-200a-3p分类
医药卫生引用本文复制引用
任华,郝强,郭丽,郑素华,卫雷..miR-200a-3p对新生大鼠缺氧缺血性脑损伤的作用和机制[J].医学分子生物学杂志,2025,22(4):339-345,7.基金项目
河北省医学科学研究课题(No.20220520) This work was supported by a grant from the Medical Science Research Project of Hebei Province(No.20220520) （No.20220520）
