医学分子生物学杂志2025，Vol.22Issue(4)：354-360,7.DOI:10.3870/j.issn.1672-8009.2025.04.008
miR-107通过ITGA2促进肺动脉平滑肌细胞凋亡在肺动脉高压中的作用研究
Effect of miR-107 and ITGA2 on Apoptosis of Pulmonary Artery Smooth Muscle Cells in Pulmonary Arterial Hypertension
摘要
Abstract
Objective To investigate the effect of miR-107 and integrin alpha 2(ITGA2)on pulmonary artery smooth muscle cells(PASMCs)in pulmonary arterial hypertension(PAH).Methods Pulmonary artery smooth muscle cells(PASMCs)were cultured under normal and hy-poxic conditions.qRT-PCR was applied to detect the expression levels of miR-107 and ITGA2 in the cells.Flow cytometry was used to determine cell cycle and apoptosis.The targeting relationship be-tween miR-107 and ITGA2 was verified by luciferase reporter gene assay.PASMCs were transfected with si-NC and si-miR-107 and cultured under hypoxic conditions,and were divided into the si-NC group and the si-miR-107 group.The expression levels of miR-107 and ITGA2,the cell cycle,and apoptosis were examined.Thirty C57BL/6 mice were divided into 3 groups:control group,model group,and si-miR-107 treatment group,with the latter two groups injected with si-NC and si-miR-107 after inducing a PAH model through hypoxia,respectively.The right ventricular hypertrophy in-dex(RVHI),right ventricular systolic pressure(RVSP),and mean pulmonary arterial pressure(mPAP)were measured.The expression levels of miR-107 and ITGA2,and phosphorylated histone H2AX(γH2AX)protein in the mouse lung tissues were detected by qRT-PCR and Western blot-ting.Results Compared with those of cells in the normal group,the expression level of miR-107 in the hypoxic group cells was increased,the ITGA2 expression level was decreased,the proportion of cells in the G1 phase was increased,the proportion of cells in the S phase was decreased,and the apoptosis rate was increased.miR-107 targeted ITGA2.Compared with those in the si-NC group,the expression level of miR-107 in the si-miR-107 group was decreased,the ITGA2 expression level was increased,the proportion of cells in the G1 phase was decreased,the proportion of cells in the S phase was increased,and the apoptosis rate was decreased.In the mice experiments,compared with those in the control group,the values of RVHI,RVSP,and mPAP,the expression level of miR-107 and γH2AX protein was increased,and the ITGA2 expression level was decreased in the model group.Compared with those in the model group,the values of RVHI,RVSP,and mPAP,the expression levels of miR-107 and γH2AX protein were decreased in the si-miR-107 treatment group,while the expression level of ITGA2 was increased.Conclusion miR-107 affects PAH by inhibiting the cell cycle and promoting the apoptosis of PASMCs through ITGA2.关键词
肺动脉平滑肌细胞/肺动脉高压/miR-107/整合素α2Key words
pulmonary artery smooth muscle cells/pulmonary arterial hypertension/miR-107/integrin alpha-2分类
医药卫生引用本文复制引用
巫凤苹,石宗民,张媛梅..miR-107通过ITGA2促进肺动脉平滑肌细胞凋亡在肺动脉高压中的作用研究[J].医学分子生物学杂志,2025,22(4):354-360,7.基金项目
四川青年创新科研课题计划项目(No.Q20033) This work was supported by a grant from the Sichuan Youth Innovation and Research Project(No.Q20033) （No.Q20033）
