医学分子生物学杂志2025，Vol.22Issue(4)：361-367,7.DOI:10.3870/j.issn.1672-8009.2025.04.009
丹参酮ⅡA通过MALAT1靶向miR-143调控结肠癌SW480细胞增殖、侵袭和迁移
Tanshinone ⅡA Regulates Proliferation,Invasion,and Migration of Colon Cancer SW480 Cells by Targeting miR-143 via MALAT1
摘要
Abstract
Objective To investigate the effect of tanshinone ⅡA on proliferation,invasion and migration of colon cancer SW480 cells by targeting miR-143 via metastasis-associated lung ade-nocarcinoma transcript 1(MALAT1).Methods SW480 cells were treated with 0,10,20,and 40 μmol/L of tanshinone ⅡA,and were randomly divided into 4 groups:Control group,tanshi-none Ⅱ A low-,medium-,and high-dose groups.CCK8 assay was used to detect cell proliferation.Wound-healing and Transwell assay were used to detect the number of migrated and in-vaded cells.The expression levels of MALAT1 and miR-143 mRNA were detected by RT-PCR.The luciferase gene reporter assay was used to verify the targeting relationship between MALAT1 and miR-143.SW480 cells treated with tanshinone ⅡA(40 μmol/L)were transfected with pcDNA-MALAT1 and miR-143 mimic separately or combined,and were randomly divided into 5 groups:Control group,PCDNA 3.1 group,pcDNA-MALAT1 group,MALAT1+mimic NC group,and MALAT1+miR-143 mimic group.The proliferation,invasion,and migration of cells in the above groups were detected.Results Compared with those in the Control group,the cell proliferation,number of invaded cells,wound-healing rate,and MALAT1 mRNA expression level in the tanshi-none ⅡA medium-dose and high-dose groups were significantly decreased(P＜0.05),and the miR-143 mRNA expression level was significantly increased(P＜0.05).MALAT1 has a targeting relationship with miR-143.Compared with those in the pcDNA-MALAT1 group,the cell prolifera-tion,number of invaded cells,and wound-healing rate in the MALAT1+miR-143 mimic group were significantly decreased(P＜0.05).Conclusion Tanshinone ⅡA exerts its anti-colon canc-er effect through theMALAT1/miR-143 axis.关键词
结肠癌/丹参酮ⅡA/肺癌转移相关转录本1/miR-143Key words
colon cancer/tanshinone ⅡA/metastasis-associated lung adenocarcinoma tran-script 1/miR-143分类
医药卫生引用本文复制引用
李鹏,杜锐,谢福平,李锦涛,王杰..丹参酮ⅡA通过MALAT1靶向miR-143调控结肠癌SW480细胞增殖、侵袭和迁移[J].医学分子生物学杂志,2025,22(4):361-367,7.基金项目
福建省医学科研计划项目(No.2023D034) This work was supported by a grant from the Fujian Medical Research Plan Project(No.2023D034) （No.2023D034）
