医学分子生物学杂志2025，Vol.22Issue(4)：368-373,6.DOI:10.3870/j.issn.1672-8009.2025.04.010
circCSE1L调控miR-16-5p对非小细胞肺癌NCI-H1299细胞转移活性的影响
Effect of circCSE1L on the Metastatic Activity of Non-small Cell Lung Cancer NCI-H1299 Cells by Regulating MiR-16-5p
摘要
Abstract
Objective To investigate the effect of circCSE1L on the metastatic activity of non-small cell lung cancer NCI-H1299 cells by regulating miR-16-5p.Methods The expression level of miR-16-5p and circCSE1L in cancer tissues and adjacent tissues of patients with non-small cell lung cancer was analyzed by real-time quantitative PCR.NCI-H1299 cells were divided into 4 groups:si NC group,si CSE1L group,miR NC group,and miR-16-5p mimic group.The proliferation activity of cells was analyzed by MTT assay.The invasion and migration abilities of cells were analyzed by Transwell assay and wound-healing assay.The apoptosis rate of cells was analyzed by flow cytome-try.Luciferase reporter gene assay was used to analyze the targeting relationship between circCSE1L and miR-16-5p in NCI-H1299 cells.Results Compared with those in the adjacent tissues,cir-cCSE1L was highly expressed in the NSCLC tissues,while miR-16-5p was lowly expressed in the NSCLC tissues.Compared with those in the si NC group,the cell proliferation activity,invasion and migration abilities in the si CSE1L group were decreased,and the apoptosis rate was in-creased.Compared with those in the miR NC group,the cell proliferation activity,invasion and mi-gration abilities of in the miR-16-5p mimic group were decreased,and the apoptosis rate was in-creased.The luciferase activity was decreased after transfection with miR-16-5p mimic in the CSE1L-WT group compared with that in the miR NC group.Conclusion circCSE1L was highly expressed in NSCLC,while miR-16-5p was lowly expressed.circCSE1L affects the proliferation,migration,and invasion of non-small cell lung cancer NCI-H1299 cells by inhibiting the expression of miR-16-5p,and promotes the apoptosis of non-small cell lung cancer cells.关键词
circCSE1L/非小细胞肺癌/转移/NCI-H1299细胞/miR-16-5pKey words
circCSE1L/non-small cell lung cancer/metastasis/NCI-H1299 cells/miR-16-5p分类
医药卫生引用本文复制引用
刘瑛,张勇,李秋波,黄念..circCSE1L调控miR-16-5p对非小细胞肺癌NCI-H1299细胞转移活性的影响[J].医学分子生物学杂志,2025,22(4):368-373,6.基金项目
2023年度荆门市重点科技计划项目(No.2023YFZD008) This work was supported by a grant from the Key Science and Technology Plan Project of Jingmen(No.2023YFZD008) （No.2023YFZD008）
