广西科学院学报2025，Vol.41Issue(2)：170-181,12.DOI:10.13657/j.cnki.gxkxyxb.20250626.001
钩藤CCD基因家族全基因组鉴定及表达模式分析
Whole Genome Identification and Expression Pattern of CCD Gene Family in Uncaria rhynchophylla
摘要
Abstract
In order to understand the expression characteristics of the Carotenoid Cleavage Dixoygenase(CCD)gene family in Uncaria rhynchophylla and its potential role in branch regulation,this study used bioinformat-ics methods to screen and identify the members of the CCD gene family in the whole genome of U.rhyncho-phylla based on the sequence information of Arabidopsis thaliana CCD gene family.The phylogenetic evolu-tion,physicochemical properties,functional structure,conserved motifs and promoter cis-acting elements were analyzed,and the expression patterns of CCD gene in different tissues were analyzed by transcriptome data.The results showed that:(1)A total of 20 CCD gene family members were identified in U.rhynchophylla,all of which had typical Retinal Pigment Epithelium Specific Protein(RPE65)domain,of which 18 were accu-rately located on 8 chromosomes.Combined with phylogenetic tree analysis,17 CCD gene family members can be divided into two categories(CCD,NCED),belonging to seven subfamilies(CCD1,CCD4,CCD7,CCD8,NCED3,NCED6,NCED9).(2)The CCD promoter region of U.rhynchophylla had cis-acting ele-ments closely related to biological processes such as stress,hormone response and growth and development.(3)UrCCD4-band UrCCD1-c were highly expressed in the leaf tissue of U.rhynchophylla,and UrCCD7-b and UrCCD8-a were highly expressed in roots and stems.Compared with the two-branched plants,the ex-pression levels of UrCCD1-b,UrCCD8-a and UrCCD8-b in the stem nodes of the three-branched plants were significantly down-regulated.The CCD gene family members of U.rhynchophylla have obvious tissue ex-pression specificity.UrCCD1-b,UrCCD8-a and UrCCD8-b play an important role in the regulation of U.rhynchophylla branching,which lays a foundation for further study of the CCD gene family of U.rhyncho-phylla.关键词
钩藤/类胡萝卜素裂解双加氧酶/基因家族/基因表达Key words
Uncaria rhynchophylla/carotenoid cleavage dioxygenase/gene family/gene expression分类
生物科学引用本文复制引用
李玉琼,张占江,韦树根,万凌云,沈洁,李林轩,冯长存,梁露,梁文静,杨翠红,潘丽梅..钩藤CCD基因家族全基因组鉴定及表达模式分析[J].广西科学院学报,2025,41(2):170-181,12.基金项目
广西科技重大专项项目(桂科AA22096021),国家自然科学基金项目(82360753),广西自然科学基金项目(2019GXNSFBA185026),广西特色药材关键技术研究推广项目(GZKJ2314),广西中医药多学科交叉创新团队项目(GZKJ2305)和广西(青年)岐黄学者培养项目(GXQH202402)资助. （桂科AA22096021）
