广西科学院学报2025，Vol.41Issue(2)：232-245,14.DOI:10.13657/j.cnki.gxkxyxb.20250722.009
Fe(Ⅱ)/α-酮戊二酸依赖型卤化酶作为生物催化剂的最新研究进展
Recent Advances of Fe(Ⅱ)/α-ketoglutarate-dependent Haloge-nases as Biocatalysts
摘要
Abstract
Carbon hydrogen bond(C-H bonds)activation by biocatalysis plays a key role in the synthesis of pharmaceuticals and fine chemicals,and the highly selective construction of carbon halogen bond(C-X bonds)is an important research direction.Although significant progress has been made in this field,the se-lective halogenation of unactivated C(sp3)-H bonds still faces multiple challenges such as chemical inert-ness breakthrough,regional and stereo-control.Fe(Ⅱ)/α-ketoglutarate(α-KG)-dependent halogenase can a-chieve precise halogenation of carbon centers in unactivated C(sp3)-H bonds under mild conditions through a unique free radical reaction mechanism,showing excellent regional,chemical and stereo-selectivity,provi-ding an innovative tool for pharmaceutical synthesis.Fe(Ⅱ)/α-KG-dependent halogenases include carrier protein type and independent type,and their halogenation reactions in the biosynthesis of natural products such as alkaloids,amino acids and nucleotides have attracted much attention.In this paper,the basic charac-teristics,catalytic mechanism and application research progress of Fe(Ⅱ)/α-KG-dependent halogenases are reviewed.The potential of using bioinformatics methods such as gene mining to discover novel Fe(Ⅱ)/α-KG-dependent halogenases is also discussed.The aim is to provide a valuable reference for exploring the applica-tion of Fe(Ⅱ)/α-KG-dependent halogenase as a biocatalyst in the synthesis of novel halides.关键词
Fe(Ⅱ)/α-酮戊二酸依赖型卤化酶/生物催化剂/卤化反应/生物合成Key words
Fe(Ⅱ)/α-ketoglutarate-dependent halogenase/biocatalyst/halogenation reaction/biosynthesis分类
生物科学引用本文复制引用
梁敏,黄媛林,罗月宁,杨登峰..Fe(Ⅱ)/α-酮戊二酸依赖型卤化酶作为生物催化剂的最新研究进展[J].广西科学院学报,2025,41(2):232-245,14.基金项目
国家自然科学基金项目(32360244),广西科技基地和人才专项(桂科AD23023007),广西科学院基本科研业务费项目(2024YWF2114,CQ-C-202302)资助. （32360244）
