南方农业学报2025，Vol.56Issue(5)：1403-1413,11.DOI:10.3969/j.issn.2095-1191.2025.05.005
分子标记辅助选择聚合Pi2和Xa23基因改良广恢1002抗病性
Improvement of Guanghui 1002 resistance by polymerizing Pi2 and Xa23 genes through molecular marker-assisted selection
摘要
Abstract
[Objective]The resistance to rice blast and bacterial blight of Guanghui 1002 was improved by polymeri-zing the Pi2 and Xa23 resistance genes,providing technical reference for maintaining elite restorer lines and further explo-ring the potential of strong dominant hybrid rice combinations.[Method]Using Guanghui 1002—the most widely culti-vated late-maturing,weak-photosensitive restorer line in southern China—as the recipient parent,simultaneously intro-duced the blast resistance gene Pi2 and bacterial blight resistance gene Xa23 via molecular marker-assisted selection(MAS)combined with conventional breeding.Five improved resistant lines were selected for disease resistance evalua-tion,agronomic trait assessment and grain quality analysis.These lines were then crossed with Jifeng A to analyze their heterosis performance.[Result]Based on molecular marker detection and field agronomic performance of isolated popula-tions,5 homozygous resistant improved lines(R1002-1,R1002-2,R1002-3,R1002-4 and R1002-5)were selected from the BC4F4 population.The 5 homozygous resistant improved lines exhibited over 97.62％genetic similarity with Guanghui 1002,with no significant difference(P>0.05)in major agronomic traits(spike length,effective spike number,grain num-ber per spike,seed setting rate,1000-grain weight and yield).However,their blast resistance frequency improved from 61.5％to 79.5％-94.9％,and bacterial blight resistance was greatly enhanced,showing high resistance to types Ⅲ,Ⅳ,Ⅴ and Ⅸ of Xanthomonas oryzae pv.oryzae.Notably,R1002-1 and R1002-3 demonstrated improved grain quality,reaching the"Ministry Standard Grade 2"level.When the 5 homozygous resistant improved lines crossed with Jifeng A,the F1 hy-brids showed no significant differences in agronomic traits(plant height,spike length,effective spike number,grain num-ber per spike,seed setting rate and yield),and the rice quality was ordinary.[Conclusion]The genetic background reco-very of 5 Guanghui 1002 improved lines resistant to rice blast and bacterial blight has met expectations.Without reducing production,the resistance of each improved line to rice blast and bacterial blight has greatly increased,and the rice qua-lity of some improved lines has improved,achieving the improvement goal.Subsequently,these lines can replace the existing Guanghui 1002 to develop hybrid rice varieties with better resistance to rice blast and bacterial blight.关键词
分子辅助标记选择/弱感光恢复系/抗稻瘟病/抗白叶枯病/育种Key words
molecular marker-assisted selection/restorer line with weak photosensitivity/rice blast resistancet/bac-terial blight resistance/breeding分类
农业科技引用本文复制引用
孔乐,柳武革,徐琳,姜文静,于咏梅,符恭震,李金华,霍兴,刘迪林,王丰..分子标记辅助选择聚合Pi2和Xa23基因改良广恢1002抗病性[J].南方农业学报,2025,56(5):1403-1413,11.基金项目
广东省重点领域研发计划项目(2022B0202060002) （2022B0202060002）
广东省农业科学院水稻研究所"优谷计划"项目(2022YG06) （2022YG06）
广东省水稻育种新技术重点实验室项目(2023B1212060042) Guangdong Key Field Research and Development Plan Project(2022B0202060002) （2023B1212060042）
"Elite Rice Plan"of Rice Research Institute of Guangdong Academy of Agricultural Sciences(2022YG06) （2022YG06）
Project of Guangdong Key Laboratory of New Technology in Rice Breeding(2023B1212060042) （2023B1212060042）
