南方农业学报2025，Vol.56Issue(5)：1431-1445,15.DOI:10.3969/j.issn.2095-1191.2025.05.007
光敏色素类互作因子OsPIL1敲除对籼稻稻瘟病抗性的影响
Effects of phytochrome-interacting factor OsPIL1 knockout on blast disease resistance in indica rice
摘要
Abstract
[Objective]To clarify the effects of knockout of phytochrome-interacting factor gene(OsPIL1)in rice(Oryza sativa)on the growth,yield and disease resistance of indica rice Yuelianggu,and to preliminarily explore effects of exogenous jasmonic acid(JA)or salicylic acid(SA)on the regulation of rice blast resistance by OsPIL1,and to ex-plore the role of OsPIL1 integrating JA/SA in regulating rice blast resistance,which could provide basic data for analy-zing the mechanism of rice blast resistance.[Method]Rice variety Yuelianggu was as material CRISPR/Cas9 technology was used to obtain OsPIL1 knockout transgenic rice line,which was analyzed for phenotypic analysis(root length,coleo-ptile length,plant height,tiller number,spike length,number of solid grains per spike,100-grain weight,grain length,grain width)and assay of rice blast resistance(observation of blast symptom and infection process)in inoculated rice plants,as well as for the analysis of rice blast resistance in inoculated rice plants after exogenous JA/SA treatment.[Result]Three pure OsPIL1 knockout transgenic rice lines(ΔOspil1 ko#7,#33 and#36)were acquired by CRISPR/Cas9.These OsPIL1 knockout transgenic rice lines exhibited lower in various agronomic traits,including plant height,til-ler number,root length,coleo-ptile length,distance from the stem base to the new leaf sheath junction,panicle length,grain number per panicle,length from the panicle node to the top leaf sheath junction,internode length,100-grain weight,grain length and grain width than those of the wild type.Compared with the wild type,the activity of peroxidase(POD)in the OsPIL1 knockout transgenic rice lines changed slightly,but the content of reactive oxygen species(ROS)increased significantly while the content of lignin decreased significantly(P<0.05,the same below),and the expression levels of chloroplasts and defense related genes in rice changed.The symptoms of rice blast in OsPIL1 knockout trans-genic rice lines infected with Magnaporthe oryzae were aggravated.Compared with the wild type inoculated with Magna-porthe oryzae,the disease index of OsPIL1 knockout transgenic rice lines was significantly increased,the number of cal-lose changed slightly,the number of dead cells and ROS content increased,the activity of POD and the content of lignin decreased,and the expression level of defense related genes decreased.Inoculation with live injury caused by Magna-porthe oryzae into OsPIL1 knockout rice lines led to a significant increase in the relative growth of fungi in individual le-sions,further confirming the reduced resistance to rice blast in OsPIL1 knockout transgenic rice lines.Exogenous JA or SA could greatly enhance the resistance of OsPIL1 knockout transgenic rice lines to rice blast,but the improvement effect was inferior to that of JA or SA on the resistance to rice blast in wild type rice.[Conclusion]OsPIL1 knockout negatively affects rice growth,grain development and rice blast resistance,and OsPIL1 regulates JA or SA pathway to improve rice blast resistance in indica rice.关键词
水稻/抗瘟性/OsPIL1/茉莉酸/水杨酸/防御响应Key words
rice/blast resistance/OsPIL1/jasmonic acid(JA)/salicylic acid(SA)/defense response分类
农业科技引用本文复制引用
左如斌,钟爰爰,杨淑敏,刘崇兰,刘娜,杨丽清,杨静..光敏色素类互作因子OsPIL1敲除对籼稻稻瘟病抗性的影响[J].南方农业学报,2025,56(5):1431-1445,15.基金项目
国家自然科学基金项目(32160643) （32160643）
云南省中青年学术和技术带头人后备人才项目(202305AC160006) National Natural Science Foundation of China(32160643) （202305AC160006）
Yunnan Young and Middle-aged Aca-demic and Technical Leaders Reserve Talent Project(202305AC160006) （202305AC160006）
