南方农业学报2025，Vol.56Issue(6)：1739-1751,13.DOI:10.3969/j.issn.2095-1191.2025.06.005
南方白甲鱼转录组学性别差异及其生物学功能解析
Sex differences and biological functions in transcriptomics of Onychostoma gerlachi
摘要
Abstract
[Objective]This study aimed to reveal differences in gene expression and metabolic pathways between fe-male and male Onychostoma gerlachi through transcriptomic analysis,which could provide reference for the exploitation and utilization of its germplasm resources.[Method]Transcriptome sequencing of mixed brain,liver,and muscle tissue samples from adult female and male Onychostoma gerlachi was performed using the Illumina HiseqTM 2000 platform.After quality control and assembly,unigenes were functionally annotated against the Nr,COG/KOG,KEGG and SWISS-PROT databases.Differentially expressed genes(DEGs)were screened using thresholds of FDR≤0.05,|log2 Fold Change|≥1 and P<0.05.Biological functions and metabolic pathways of DEGs were analyzed via GO functional annota-tion and KEGG pathway enrichment.SSR loci were identified using MISA.[Result]Transcriptome sequencing of 6 Ony-chostoma gerlachi tissue samples generated 59628 unigenes,with N50 length of 2209 bp.The annotation success rate across Nr,COG/KOG,KEGG and SWISS-PROT databases was 55.82%,with Nr annotating the most unigenes(33201),and 71.06%annotated genes was homologous to zebrafish.A total of 3116 DEGs were identified between male and female Onychostoma gerlachi:2254 down-regulated and 862 up-regulated.Among the top 10 up-regulated DEGs,4 were unannotated and exclusively expressed in male samples.DEGs were primarily enriched in GO terms including bio-logical process,metabolic process,cellular process,binding and catalytic activity.KEGG metabolic pathways enrich-ment revealed 614 DEGs enriched to 158 pathways,covering lipid metabolism,amino acid metabolism,carbohydrate metabolism,endocrine system,and transport/catabolism.A total of 12012 SSR loci were screened out from the transcrip-tome of Onychostoma gerlachi,among which dinucleotide type SSR loci were the most numerous(6740),accounting for 56.11%.[Conclusion]There are a large number of DEGs in the transcriptomes of female and male Onychostoma gerla-chi,which leads to ob-vious differences in the utilization ability and strategies of different nutrient metabolisms between female and male indivi-duals,with females exhibiting potentially higher metabolic activity.Thus,future research on growth performance and stress resistance in Onychostoma gerlachi should consider sex-specific metabolic variations.关键词
南方白甲鱼/差异表达基因(DEGs)/性别差异/代谢通路/SSR位点Key words
Onychostoma gerlachi/differentially expressed genes(DEGs)/sex differences/metabolic pathway/SSR loci分类
农业科技引用本文复制引用
王元浩,廖愚,王培培,肖珊,张盛,唐永凯,黄彩林,张志新,郑海涛,曹寿雄..南方白甲鱼转录组学性别差异及其生物学功能解析[J].南方农业学报,2025,56(6):1739-1751,13.基金项目
国家重点研发计划项目(2022YFF0608200) （2022YFF0608200）
广西农业科技自筹经费项目(Z202259) National Key Research and Development Program of China(2022YFF0608200) （Z202259）
Guangxi Agri-cultural Science and Technology Self-financing Project(Z202259) （Z202259）
