摘要
Abstract
The article aims to increase the butanol production in Escherichia coli through metabolic engineering,and enhance the sustainability of bioenergy.Based on systems biology analysis,the metabolic network of E.coli was engineered,including the overexpression of key enzymes,inhibition of by-product pathways,and introduction of new metabolic pathways.Growth and metabolites were monitored using HPLC and GC.The engineered strain produced butanol at 7.50±0.30 g/L and 12.20±0.45 g/L after 24 and 48 hours,respectively,with production rates of 0.31±0.01 g/(L·h)and 0.25±0.01 g/(L·h),respectively.Meanwhile,cell growth and glucose consumption were accelerated,with by-products including acetate,lactate,and ethanol.The metabolic engineering strategy effectively increased butanol production and productivity,which provides a new avenue for bioenergy production.关键词
代谢工程/大肠杆菌/丁醇产量/系统生物学/生物能源Key words
metabolic engineering/Escherichia coli/butanol production/systems biology/bioenergy
