工业微生物2025，Vol.55Issue(3)：29-32,4.DOI:10.3969/j.issn.1001-6678.2025.03.010
6-甲基香豆素抑菌活性及抑菌稳定性研究
Study on Antifungal Activity and Stability of 6-methylcoumarin
摘要
Abstract
The purpose of this study was to explore the antifungal activity and stability of 6-methylcoumarin on Botrytis cinerea.In this study,Botrytis cinerea was selected as the research object.The growth rate method was used to determine the antifungal activity of 6-methylcoumarin against Botrytis cinerea hyphae in vitro.The antifungal activity of 6-methylcoumarin against Botrytis cinerea under different conditions was further determined.The results showed that different concentrations of 6-methylcoumarin had certain antifungal activity against Botrytis cinerea,showing a linear relationship between dose and inhibition rate.With the gradual increase of treatment temperature,the antifungal activity gradually decreased.When the treatment temperature reached 60 ℃,the inhibition rate was 23.40±4.05％,and the antifungal activity was significantly reduced(P＜0.05).With the extension of ultraviolet irradiation time,the antifungal activity of 6-methylcoumarin against Botrytis cinerea was gradually enhanced.When the irradiation time reached 12 h,the inhibition rate was 73.86±2.23％,which was significantly higher than that of the control group(P＜0.05).Different types of metal ions had different effects on the antifungal activity of 6-methylcoumarin.Among them,the antifungal effect of 6-methylcoumarin was significantly enhanced after treatment with Na+and Ca2+(P＜0.05).After K+treatment,the antifungal activity of 6-methylcoumarin remained stable.When the pH was 6-9,the antifungal activity of 6-methylcoumarin against Botrytis cinerea remained stable.This study can provide a theoretical basis for the use of 6-methylcoumarin in the control of gray mold.关键词
6-甲基香豆素/灰葡萄孢菌/生长速率法/抑菌活性/抑菌稳定性Key words
6-methylcoumarin/Botrytis cinerea/growth rate method/antifungal activity/antifungal stability引用本文复制引用
陈运泽,王增平,陈艳,宋玫,罗倩,张国财..6-甲基香豆素抑菌活性及抑菌稳定性研究[J].工业微生物,2025,55(3):29-32,4.基金项目
贵州省教育厅自然科学研究项目(青年项目)(黔教技[2022]259号) （青年项目）
贵州省林业局2024年度林业科学技术研究项目(黔林科合[2024]21号) （黔林科合[2024]21号）
东北林业大学与贵州师范学院2024年度研究生专项科研基金项目(编号:2024YJS07) （编号:2024YJS07）
贵州师范学院大学生自主科研项目(编号:2023DXS022). （编号:2023DXS022）
