贵州农业科学2025，Vol.53Issue(7)：81-89,9.DOI:10.3969/j.issn.1001-3601.2025.07.009
基于线粒体D-loop区和COI基因序列的毕节地区2种裂腹鱼遗传多样性分析
Genetic Diversity Analysis of Two Schizothorax Species in Bijie Region Based on Mitochondrial D-loop and COI Gene Sequences
摘要
Abstract
[Objective]The genetic diversity of Schizothorax grahami and Schizothorax kozlovi in Bijie of Guizhou was investigated to provide a reference for germplasm conservation and stock enhancement.[Method]Mitochondrial D-loop region and COI gene sequences were used as molecular markers to analyze genetic diversity of two Schizothorax species.[Result]10 and 9 haplotypes were defined based on mitochondrial D-loop and COI gene sequence respectively in the 38 individuals of S.grahami,meanwhile the haplotype diversity(Hd)and nucleotide diversity(π)were 0.781,0.630 and 0.006 44,0.003 73 respectively.5 and 4 haplotypes were defined based on mitochondrial D-loop and COI gene sequence respectively in the 34 individuals of S.kozlovi,meanwhile the haplotype diversity(Hd)and nucleotide diversity(π)were 0.524,0.485 and 0.002 62,0.001 21 respectively.The neutral detection and nucleotide mismatch distribution indicated that the two Schizothorax species may not have experienced large-scale population expansion events recently.[Conclusion]The genetic diversity of S.grahami is at a medium level in Bijie of Guizhou,whereas the genetic diversity of S.kozlovi is low.关键词
昆明裂腹鱼/四川裂腹鱼/D-loop区/COI基因/遗传多样性Key words
Schizothorax grahami/Schizothorax kozlovi/D-loop region/COI gene/genetic diversity分类
农业科技引用本文复制引用
刘伟,王霞,向燕,吕振宇,曾圣,詹会祥..基于线粒体D-loop区和COI基因序列的毕节地区2种裂腹鱼遗传多样性分析[J].贵州农业科学,2025,53(7):81-89,9.基金项目
贵州省农业科学院青年基金项目(黔农科院青年基金[2023]28号),(黔农科院青年科技基金[2021]14号) （黔农科院青年基金[2023]28号）
