耕作与栽培2025，Vol.45Issue(5)：22-30,9.
红果参内参基因的筛选与验证
Screening and Validation of Reference Genes in Cyclocodon lancifolius
摘要
Abstract
To investigate the optimum reference gene for gene expression analysis in different tissues and under various abi-otic stress for edible and medicinal new resource plant of Cyclocodon lancifolius(Roxb.)Kurz.In this study,five candidate internal reference genes(ACT,TUA,TUB,GAPDH,UBC)were selected based on the transcriptome data of Cyclocodon lan-cifolius.The expression levels of candidate reference genes were assessed by quantitative real-time PCR.The gene expres-sion stability analysis software was utilized to evalute the stability expression of five candidate internal reference genes of Cy-clocodon lancifolius.Subsequently,the functional genes of Cyclocodon lancifolius were used to identified and validated the optimal internal reference genes.Our findings indicated that ACT had the best expression stability in different tissues and high temperature stress,the expression stability of TUA was the best under NaCl stress,the expression stability of UBC was the best in ABA treatment and low temperature stress,the expression stability of GAPDH was the best under PEG stress.Comprehensive analysis and using SAPK1 and PP2CA as target genes to verified the screened reference genes indicated that ACT was the most suitable reference gene of Cyclocodon lancifolius in different tissues and under abiotic stresses gene ex-pression study.关键词
红果参/实时荧光定量PCR/内参基因/稳定性Key words
cyclocodon lancifolius/real-time quantitative PCR/internal reference gene/stability分类
农业科技引用本文复制引用
赵永琪,杨欢欢,王健,孙庆文,陈敬忠,陈春伶,陈亮,黄园..红果参内参基因的筛选与验证[J].耕作与栽培,2025,45(5):22-30,9.基金项目
贵州省大学生创新创业训练计划项目(贵中医大创合字[2023]154号) （贵中医大创合字[2023]154号）
贵州省高层次创新型人才培养计划项目(黔科合平台人才-GCC[2023]077) （黔科合平台人才-GCC[2023]077）
贵州省科技计划项目(黔科合基础-ZK[2022]一般458) （黔科合基础-ZK[2022]一般458）
