海南医科大学学报2025，Vol.31Issue(13)：970-978,9.DOI:10.13210/j.cnki.jhmu.20240927.003
鸡血藤提取物通过PI3K/AKT通路对缺铁性贫血大鼠的药效和作用机制研究
Efficacy and mechanism of Spatholobus suberectus extract on iron deficiency anemia rats via PI3K/AKT pathway
摘要
Abstract
Objective:To investigate the efficacy and mechanism of Spatholobus suberectus(S.suberectus)extract on iron defi-ciency anemia(IDA)rats.Methods:A low iron diet combined with cutting the tail and bleeding was used to construct an IDA rat model.The efficacy of S.suberectus extract was evaluated by measuring peripheral blood count,serum iron,total iron binding ca-pacity,transferrin saturation,and serum ferritin levels,observing morphological changes in liver and kidney tissues,and assessing the levels of oxidative stress markers and hepcidin in the liver.Furthermore,network pharmacology methods were used to screen potential pharmacodynamic targets and key signaling pathways,and the important pharmacodynamic targets in key signaling path-ways in the liver were verified by reverse transcription-real-time quantitative polymerase chain reaction(RT-qPCR)and Western blot to reveal their mechanism of action.Results:S.suberectus extract significantly improved peripheral blood count indices such as red blood cell count,hemoglobin concentration,hematocrit,and mean corpuscular volume,mean corpuscular hemoglobin,and mean corpuscular hemoglobin concentration,and slowed down the decreasing rate of serum iron and transferrin saturation in IDA rats(P<0.05),repaired the damage of kidney and liver tissues,and increasing the content of hepcidin(P<0.01)and antioxidant capacity in liver tissues.At the molecular level,S.suberectus extract upregulated the expression of PI3K and AKT mRNA,as well as the levels of PI3K,p-PI3K,AKT and p-AKT proteins.Conclusion:S.suberectus extract can promote the recovery of pe-ripheral blood count,regulate serum iron metabolism level,repair liver and kidney tissue damage,and increase hepcidin content and antioxidant capacity in IDA rats.These improvements may be achieved by activating the PI3K/AKT signaling pathway in liv-er tissues.关键词
鸡血藤提取物/缺铁性贫血大鼠/药效/作用机制/PI3K/AKT信号通路Key words
Spatholobus suberectus extract/Iron deficiency anemia rats/Efficacy/Mechanism/PI3K/AKT signaling path-way分类
医药卫生引用本文复制引用
侯岚,杨彩艳,李保生,韦宝敏,黄艳新,黎飚,方晓燕,黄永毅,王金花,罗凯杰..鸡血藤提取物通过PI3K/AKT通路对缺铁性贫血大鼠的药效和作用机制研究[J].海南医科大学学报,2025,31(13):970-978,9.基金项目
This study was supported by the Key Research and Development Program of Guangxi(GUIKEAB1850004),the 2021 Annual Research Projects of Youjiang Medical University for Nationalities(yy2021sk018)and Scientific Research and Technology Development Pplan of Baise(BAIKE20211803-1) 广西重点研发计划(桂科AB1850004) （GUIKEAB1850004）
右江民族医学院2021年度校级科研课题(yy2021sk018) （yy2021sk018）
百色市科学研究与技术开发计划(百科20211803-1) （百科20211803-1）
