海南医科大学学报2025，Vol.31Issue(13)：979-985,992,8.DOI:10.13210/j.cnki.jhmu.20240927.004
木犀草素通过AKT/GSK3β/β-catenin通路对人肝癌细胞Hep3B增殖侵袭转移的影响及机制
Effect and mechanism of luteolin on the proliferation,invasion and metastasis of human hepatoma cell Hep3B through the AKT/GSK3β/β-catenin pathway
摘要
Abstract
Objective:To investigate the impact and underlying mechanisms of luteolin on the proliferation,invasion,metasta-sis,and epithelial-mesenchymal transition(EMT)of human liver cancer cell line Hep3B.Methods:The Hep3B liver cancer cell line was divided into control and luteolin treatment groups,including low,medium,and high concentrations of luteolin,as well as a high concentration combined with an AKT agonist.Cell proliferation activity was assessed using a CCK8 experiment,while mi-gration and invasion abilities were measured through scratch and Transwell assays,respectively.Fluorescence and Western blot experiments were employed to detect EMT-related proteins and examine the AKT/GSK3β/β-catenin signaling pathway.Nude mouse tumorigenesis experiments were conducted to observe tumor formation and weight,with immunohistochemistry used to as-sess Ki67 and EMT related proteins.Results:Luteolin inhibited the activity of Hep3B liver cancer cells in a dose-dependent man-ner,with higher concentrations demonstrating more pronounced effects on invasion and metastasis.Luteolin suppressed the levels of pAKT,pGSK3β,and β-catenin proteins,as well as the expression of N-cadherin,vimentin,and MMP-2,while increasing E-cadherin expression.The AKT agonist SC79 was found to counteract the impact of high luteolin concentrations on tumor cells.Conclusion:Luteolin may impede the proliferation,invasion,metastasis,and EMT of Hep3B hepatocellular carcinoma cells via the AKT/GSK3β signaling pathway.关键词
木犀草素/AKT/GSK3β/β-catenin/肝癌/增殖/迁移/转移/EMTKey words
Luteolin/AKT/GSK3β/β-catenin/Hepatoma/Proliferation Migration/Metastasis/Epithelial-mesenchymal tran-sition分类
医药卫生引用本文复制引用
骆泽潭,赵睿,张银亮,顾林,赵娜,李大鹏,奥迪,郑海伦..木犀草素通过AKT/GSK3β/β-catenin通路对人肝癌细胞Hep3B增殖侵袭转移的影响及机制[J].海南医科大学学报,2025,31(13):979-985,992,8.基金项目
This study was supported by the Anhui Province Higher Education Institutions Scientific Research Project(Natural Science)(2022AH040216,2022AH051489,2023AH0519542)and Bengbu Medical University Natural Science Research Project(2020byzd170) 安徽省高等学校科学研究项目(自然科学类)(2022AH040216、2022AH051489、2023AH0519542) （Natural Science）
蚌埠医科大学自然科学研究项目(2020byzd170) （2020byzd170）
