海南医科大学学报2025，Vol.31Issue(13)：986-992,7.DOI:10.13210/j.cnki.jhmu.20250509.006
夹脊电针调控HMGB1/RAGE/NF-κB通路抑制脊髓损伤大鼠小胶质细胞活化的机制研究
Mechanism of Jiaji electroacupuncture inhibiting the activation of microglia in rats with spinal cord injury via regulating HMGB1/RAGE/NF-κB pathway
摘要
Abstract
Objective:To investigate the effect of Jiaji electroacupuncture at the spinal cord on the activation of microglia in rats with spinal cord injury by regulating the HMGB1/RAGE/NF-κB pathway.Methods:The SCI rat model was established us-ing Allen's method,and the rats were randomly divided into a sham surgery group,a model group,and an electroacupuncture group.BBB score was used to evaluate hind limb motor function in rats after SCI;ELISA method was used to detect the expres-sion of inflammatory factors;Western blot was used to detect the expression levels of HMGB1,RAGE,and NF-κB proteins;Im-munofluorescence was used to observe the expression of small glial cell marker Iba-1.Results:Compared to the sham surgery group,the BBB scores of the model group were significantly reduced at all time points,indicating successful modeling.The scores of the electroacupuncture group were significantly higher than those of the model group at 3,7,and 14 d.The expression of pro-in-flammatory factors in the electroacupuncture group was significantly reduced compared to the model group.The protein expression of HMGB1,RAGE,and p-NF-κB p65/NF-κB p65 in the electroacupuncture group was lower than that in the model group.The expression of Iba-1 in the electroacupuncture group was significantly reduced compared to the model group.Conclusion:Jiaji elec-troacupuncture can inhibit the activation of HMGB1/RAGE/NF-κB pathway in rats with spinal cord injury,thereby suppressing the activation of microglia,reducing neuroinflammation,and promoting the recovery of neurological function in rats with spinal cord injury.关键词
脊髓损伤/夹脊电针/小胶质细胞/神经炎症/HMGB1/RAGE/NF-κBKey words
Spinal cord injury/Jiaji electroacupuncture/Microglia/Neuroinflammation/HMGB1/RAGE/NF-κB分类
医药卫生引用本文复制引用
朱嘉民,桑博文,栾逸先,尹洪娜,刘莉,梁洪文..夹脊电针调控HMGB1/RAGE/NF-κB通路抑制脊髓损伤大鼠小胶质细胞活化的机制研究[J].海南医科大学学报,2025,31(13):986-992,7.基金项目
This study was supported by the Traditional Chinese Medicine Research Project of Heilongjiang Provincial Administration of Traditional Chinese Medicine(Youth Traditional Chinese Medicine Research Project)(ZHY2024-231),First Batch of Hainan Province's"Nanhai New Star"Medical and Health Talent Platform Project(NHXX-WJW-2023018),and Youth Science and Technology Innovation Ability Training Program of Heilongjiang University of Traditional Chinese Medicine(2024XJJ-QNCX024,2024-KYYWF-1393) 黑龙江省中医药管理局中医药科研课题(青年中医药科研课题)(ZHY2024-231) （Youth Traditional Chinese Medicine Research Project）
首批海南省"南海新星"医疗卫生人才平台项目(NHXX-WJW-2023018) （NHXX-WJW-2023018）
黑龙江中医药大学青年科技创新能力培养计划(2024XJJ-QNCX024,2024-KYYWF-1393) （2024XJJ-QNCX024,2024-KYYWF-1393）
