海南医科大学学报2025，Vol.31Issue(13)：993-999,7.DOI:10.13210/j.cnki.jhmu.20240918.001
骨膜水凝胶负载脂肪间充质干细胞来源外泌体治疗骨关节炎
Periosteal hydrogel-loaded adipose mesenchymal stem cell-derived exosomes for the treatment of osteoarthritis
摘要
Abstract
Objective:A periosteum extracellular matrix(PECM)hydrogel loaded with exosomes from adipose mesenchymal stem cells(AdMSC-Exos)was prepared.And its therapeutic effects on osteoarthritis rats induced by destabilization of medial me-niscus(DMM)were evaluated.Methods:AdMSC-Exos were extracted using ultrafiltration method and then characterized.PECM were preparated using decellularization and enzymatic degradation processes.The hydrogels were loaded with exosomes to obtain PECM@AdMSC-Exo.Male SD rats were randomly divided into sham group,DMM+Saline group,DMM+PECM group,DMM+AdMSC-Exo group and DMM+PECM@AdMSC-Exo group.The therapeutic effect of the drug on osteoarthritis was observed in vivo by intra-articular injection.Results:The results of characterization experiments showed that AdMSC-Exo,PECM and PECM@AdMSC-Exo were successfully preparated.In vitro experiments showed that AdMSC-Exo had a reparative effect on injured chondrocytes.Compared with the control group,PECM@AdMSC-Exo can effectively repair cartilage and had a better therapeutic effect on osteoarthritis in rats,and had no significant effect on the body weight of rats.Conclusions:PECM@AdMSC-Exo were successfully preparated,which showed better repair effects on cartilage injury.This study provides a new strategy for the treatment of osteoarthritis.关键词
骨膜细胞外基质水凝胶/间充质干细胞外泌体/软骨修复/骨关节炎Key words
Periosteal extracellular matrix hydrogel/MSC exosomes/Cartilage repair/Osteoarthritis分类
医药卫生引用本文复制引用
章竞,常恬,刘欣,张诗晗,刘阳,霍强..骨膜水凝胶负载脂肪间充质干细胞来源外泌体治疗骨关节炎[J].海南医科大学学报,2025,31(13):993-999,7.基金项目
Major Project of Anhui Provincial Department of Education(KJ2020ZD52) （KJ2020ZD52）
National College Student Innovation Training Program Project(202310367044) （202310367044）
Bengbu Medical College Graduate Research Innovation Program(Byycxz23023) 安徽省教育厅重大项目(KJ2020ZD52) （Byycxz23023）
国家级大学生创新训练计划项目(202310367044) （202310367044）
蚌埠医学院研究生科研创新计划项目(Byycxz23023) （Byycxz23023）
