海南医科大学学报2025，Vol.31Issue(13)：1007-1021,15.DOI:10.13210/j.cnki.jhmu.20241126.002
基于网络药理学和动物实验探讨六味补气方对慢性阻塞性肺疾病的干预作用
Intervention effect of compound traditional Chineses medicine Liuwei Buqi decoction in treatment of COPD based on network pharmacology and animal experiments
摘要
Abstract
Objective:To investigate the mechanism of the compound Chinese medicine of Liuwei Buqi decoction in the treat-ment of chronic obstructive pulmonary disease(COPD)based on network pharmacology,molecular docking and animal experi-ments.Methods:Using the online databases to obtain the relevant chemical ingredients,target proteins of Liuwei Buqi decoction and related disease genes,to choose the intersection genes,and to build protein interaction(PPI)network analysis by STRING da-tabases,relating targets for gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis by David data-bases.Using the AutodockTools and PyMOL to achive the molecular docking and visual process.Establishing the COPD model by lipopolysaccharide(LPS)combined with cigarette smoking.Givig Liuwei Buqi decoction to intervent and collecting samples after drug intervention.The prediction results of network pharmacology were verified by detecting the lung function,and the pathological changes of lung tissue by H&E staining and detecting the level of interleukin-10(IL-10)in lung tissue homogenate were by ELI-SA.Results:Network pharmacology analysis identified 76 active components and 768 potential targets.GO enrichment analysis obtained 884 GO entries.KEGG enrichment analysis obtained 139 signaling pathways.The molecular docking results showed that the main active ingredients of Liuwei Buqi decoction in the treatment of COPD are kaempferol,quercetin,oleic acid,β-sitosterol and nobiletin.Liuwei Buqi decoction has a good binding energy with TNF,AKT1,IL-6and IL-10,which are the core targets of COPD.Experimental results discoverd that compared with the normal control group,the lung function of the model group was de-creased(P<0.01).Chronic bronchitis and pulmonary emphysema occured in the lung tissue.The level of IL-10 was decreased(P<0.01).Compared with the model group,the lung function of each medication group was improved(P<0.01,P<0.05).The pathological changes of bronchopulmonary tissues were improved.The level of IL-10 in lung tissue was increased(P<0.01).Con-clusion:Liuwei Buqi decoction can increase lung function,relieve the pathological changes of COPD bronchial and lung tissue and inhibit the inflammatory response by multiple components,targets and signaling pathways.The suppression of inflammation factor IL-10 may play an significant role.关键词
六味补气方/慢性阻塞性肺疾病/网络药理学/动物实验/炎症Key words
Liuwei Buqi decoction/Chronic obstructive pulmonary disease/Network pharmacology/Animal experiments/Inflammation分类
医药卫生引用本文复制引用
赵晶燕,吴柏合,栾雪婧,蒋庆要,方远,刘向国..基于网络药理学和动物实验探讨六味补气方对慢性阻塞性肺疾病的干预作用[J].海南医科大学学报,2025,31(13):1007-1021,15.基金项目
This study was supported by Natural Science Foundation of Anhui Province(1708085MH222) 安徽省自然科学基金资助项目(1708085MH222) （1708085MH222）
