河南农业大学学报2025，Vol.59Issue(4)：614-624,11.DOI:10.16445/j.cnki.1000-2340.20241121.002
油茶CKX基因家族鉴定及表达分析
Genome-wide identification and expression analysis of CKX gene family in Camellia oleifera
摘要
Abstract
[Objective]Identifying the Camellia oleifera CKX gene family member within the common Ca.oleifera genome and studying the effects on the expression pattern of this family of genes during disease resistance have laid a certain foundation for further research on the molecular,mechanisms involved in the regulation of Ca.oleifera CKX genes in response to adversity.[Method]The members of Ca.oleifera CKX gene family were identified and analyzed by the whole genome data of Ca.oleif-era,including protein physicochemical property analysis,phylogenetic tree construction,gene struc-ture analysis,protein secondary structure and tertiary structure analysis,promoter cis-acting element analysis,and covariance analysis.The leaves of Ca.oleifera were inoculated with anthrax,and the expression patterns of the Ca.oleifera CKX gene family were analyzed at 12,24 and 48 d post-inoculation.[Result]The CoCKX gene family has seven members,which are divided into four sub-families according to the evolutionary relationship.Ca.oleifera has a total of 15 chromosomes,among which the Ca.oleifera CKX gene family is distributed on five chromosomes.The seven CoCKX genes are hydrophilic proteins,which are mainly localized in chloroplasts and are structurally composed of α-helices and irregular convolutions.All seven members of the gene family have exons and introns,and there are two members with no UTRs.Their proteins are more structurally similar,with a consistent base sequence.cis-acting elements showed that there were a large number of elements related to light response,salicylic acid,methyl jasmonate response and adversity response in the promoter region of the CoCKX gene family.The covariance results showed that there was one pair of tandem duplicated genes in the CoCKX gene family.The expression of seven genes was up-regulated at the three periods during disease resistance in Ca.oleifera leaves to varying degrees compared with that of the healthy plants.Notably,the up-regulated expression of the CoCKX1/2 genes was higher,which might regulate the response mechanism of the oil tea in the process of disease resistance to a greater extent.[Conclu-sion]The CoCKX gene family is functionally distinct,and the gene structure as a whole is evolution-arily highly conserved.CKX gene expression is up-regulated during disease resistance,and it is hypothesized that it has the ability to regulate the ability of leaves to withstand disease.关键词
油茶/CKX基因家族/抗病/基因表达/生物信息学Key words
Camellia oleifera/CKX gene family/disease resistance/gene expression/bioinformatics分类
农业科技引用本文复制引用
乔新派,郝文乾,叶希琛,魏子申,王文峰,付盼,赖勇,杨超臣,张党权..油茶CKX基因家族鉴定及表达分析[J].河南农业大学学报,2025,59(4):614-624,11.基金项目
国家自然科学基金项目(32171835) （32171835）
河南农业大学高层次人才专项支持基金项目(30501511) （30501511）
国家林业和草原局管理干部学院重点项目(202308) （202308）
