中医学报2025，Vol.40Issue(8)：1622-1628,7.DOI:10.16368/j.issn.1674-8999.2025.08.260
从氧化应激探讨肌萎缩侧索硬化症的发病机制及相关天然药物的筛选
Pathogenesis of Amyotrophic Lateral Sclerosis from Oxidative Stress and Screening of Related Natural Medicines
摘要
Abstract
Objective:To investigate the pathogenesis of amyotrophic lateral sclerosis(ALS)from the perspective of oxidative stress through bioinformatics,and to screen natural drugs that may intervene in ALS,to provide new ideas for the treatment of ALS.Methods:The datasets related to ALS and meeting the screening conditions were retrieved in the GEO database,and DESeq2 differential analysis was performed to obtain the differential genes of ALS.Genes associated with oxidative stress were collected in the GeneCards database.A protein-protein interaction(PPI)network was constructed to common genes for differential expression of ALS and oxidative stress.Gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis of ALS targets related to oxidative stress were performed by R software.The CMap platform was used to screen the natural active ingredients that intervened in ALS,and TCMSP was used to find the source of the natural active ingredients.Results:A total of 1 224 differential genes were obtained from the GSE124439 dataset in the GEO database.A total of 13 632 oxidative stress-related targets were collected and intersected with ALS dif-ferential genes,and imported into the STRING database to obtain the PPI network between ALS targets related to oxidative stress,and 10 core targets were screened.Enrichment analysis showed that the common targets of ALS and oxidative stress were related to inflamma-tion,cell proliferation and chemotaxis.The phospholipinositide 3-kinase/protein kinase B signaling pathway and the interaction be-tween cytokines and their specific receptors ranked high in KEGG analysis.The screening results of CMap platform showed that celand-erythrine,norporine hairline,quercetin marigold,honokiol,umbelliferol,hypericin and papaverine were the most likely natural active in-gredients for the treatment of ALS related to oxidative stress,and these active ingredients were widely derived from a variety of tradition-al Chinese medicines,and the molecular docking results showed that the binding energy of natural active ingredients and their corre-sponding targets was less than-5.0 kcal/mol.Conclusion:Oxidative stress and neuroinflammation are inseparable from the pathogene-sis of ALS,and the seven natural active ingredients screened in this article may have potential therapeutic effects on ALS with oxidative damage.关键词
肌萎缩侧索硬化症/氧化应激/天然药物筛选Key words
amyotrophic lateral sclerosis/bioinformatics/oxidative stress/screening of related natural medicines分类
医药卫生引用本文复制引用
陶紫晶,李傅尧,李婷,倪敬年,魏明清,时晶,田金洲..从氧化应激探讨肌萎缩侧索硬化症的发病机制及相关天然药物的筛选[J].中医学报,2025,40(8):1622-1628,7.基金项目
国家重点研发计划项目(2023YFC3504304) （2023YFC3504304）
北京中医药大学"解码中医"协同攻关项目(BZY-JMZY-2022-002) （BZY-JMZY-2022-002）
北京中医药大学2024年度基本科研业务费项目(2024-JYB-KYPT-07) （2024-JYB-KYPT-07）
