河海大学学报（自然科学版）2025，Vol.53Issue(4)：56-64,9.DOI:10.3876/j.issn.1000-1980.2025.04.007
异养菌强化下的黄铁矿基自养反硝化系统脱氮效果及机理
Effects and mechanisms of nitrogen removal in pyrite-based autotrophic denitrification system enhanced by heterotrophic bacteria
摘要
Abstract
In view of the limited comparative research of pyrite-based iron autotrophic denitrification and sulfur autotrophic denitrification and low efficiency in nitrogen removal,pyrite was utilized as an electron donor for both sulfur autotrophic denitrification and iron autotrophic denitrification.Moreover,their performance in removing nitrogen from wastewater with a low carbon/nitrogen ratio(C/N)and the enhancement mechanisms involving heterotrophic bacteria were compared and investigated.The results show that the pyrite-based iron autotrophic denitrification system achieves higher nitrogen removal efficiency than the sulfur autotrophic denitrification system,and adding an appropriate amount of heterotrophic bacteria can enhance the nitrogen removal effect.At a heterotrophic-to-autotrophic sludge volume ratio of 1∶1,the pyrite-based iron autotrophic denitrification system yields effluent TN and NO3--N concentrations of 0.26 and 0 mg/L after 48 h,which were significantly lower than those of the sulfur autotrophic denitrification system(3.94 and 2.61 mg/L)under identical influent conditions.Thermomonas,Geothrix,Ferritrophicum,Acidovorax,Pseudomonas,Brachymonas,and other denitrifying bacteria can use reduced iron as an electron donor to participate in denitrification,and their relative abundance in the pyrite-based iron autotrophic denitrification system is all higher than that in the sulfur autotrophic denitrification system.Furthermore,the addition of heterotrophic sludge into the system can introduce Thauera.Compared with sulfur autotrophic bacteria,iron autotrophic bacteria and heterotrophic bacteria can exert a better synergistic denitrification and nitrogen removal effect.关键词
黄铁矿/铁自养反硝化/硫自养反硝化/异养菌/低C/N污水Key words
pyrite/iron autotrophic denitrification/sulfur autotrophic denitrification/heterotrophic bacterium/wastewater with low C/N分类
资源环境引用本文复制引用
赵联芳,禹颖,薛柳莹,王莉..异养菌强化下的黄铁矿基自养反硝化系统脱氮效果及机理[J].河海大学学报（自然科学版）,2025,53(4):56-64,9.基金项目
国家重点研发计划项目(2022YFC3202702,2023YFC3208004) （2022YFC3202702,2023YFC3208004）
