黑龙江农业科学Issue(7)：35-39,5.DOI:10.11942/j.issn1002-2767.2025.07.0035
夹竹桃醇溶物对福寿螺卵的毒杀效果
Toxic Effect of Nerium oleander Alcohol-Soluble Substances on Pomacea canaliculata Eggs
摘要
Abstract
In order to develop an environment-friendly control strategy for Pomacea canaliculata,this study investigated the toxic effect and mechanism of alcohol-soluble substances from Nerium oleander on P.canaliculata eggs.Alcohol-soluble substances were prepared by ethanol extraction,and spectrophotometry showed the total flavonoid content(calculated as rutin)was 0.067 3 mg·mL-1.Treating P.canaliculata eggs with diluted alcohol-soluble extracts from N.oleander leaves revealed that the hatching rate of the treatment group was significantly lower than the control group,with the maximum inhibition rate exceeding 31.7％.Physiological and biochemical analyses indicated that the soluble protein content in the treated eggs decreased by 61.4％,the activities of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)decreased by 89.9％and 88.1％,respectively.However,the activities of superoxide dismutase(SOD)and peroxidase(POD)showed no significant changes.The results showed that the alcohol-soluble extract of Nerium oleander has certain toxic effects on the eggs of Pomacea canaliculata,inhibiting embryonic development.Meanwhile,it can significantly affect the energy metabolism pathway of eggs,destroy the antioxidant enzyme system in yolk granules,and disintegrate the defense mechanism of eggs.关键词
夹竹桃醇溶物/福寿螺卵/毒杀效应/谷丙转氨酶/可溶性蛋白质Key words
Nerium oleander alcohol-soluble extract/Pomacea canaliculata eggs/toxic effect/alanine aminotransferase(ALT)/soluble protein引用本文复制引用
蔡欣妮,刘湘铃,王丹娜,罗秋兰..夹竹桃醇溶物对福寿螺卵的毒杀效果[J].黑龙江农业科学,2025,(7):35-39,5.基金项目
2023年广东省科技创新战略专项资金(大学生科技创新培育)项目(pdjh202360343) （大学生科技创新培育）
广东省教育厅项目(2023KTSCX084) （2023KTSCX084）
韩山师范学院课程思政示范团队项目(E22049). （E22049）
