黑龙江农业科学Issue(7)：51-60,10.DOI:10.11942/j.issn1002-2767.2025.07.0051
葡萄病原真菌分离鉴定及拮抗细菌筛选
Isolation and Identification of Grape Pathogenic Fungi and Screening of Antagonistic Bacteria
摘要
Abstract
In July 2023,symptoms such as leaf edge yellowing and the presence of black spots were observed on grape leaves in a vineyard located at the Horticultural Research Institute of Qiqihar City,Heilongjiang Province,China.In order to determine the causative agents of the observed disease,this study employed a tissue isolation method to isolate and purify pathogenic fungi from naturally infected leaves.The identification of these pathogenic fungi was conducted based on their morphological characteristics,ITS-rRNA sequences,and phylogenetic analysis.The pathogenicity of the isolated strains was confirmed through a detached leaf inoculation method,and bacteria exhibiting antagonistic effects against the grape pathogenic fungi were subsequently screened.Morphological and ITS sequence analyses identified the pathogenic fungi as follows,PT9-4 was identified as Alternaria alternata,PT10-2 as Curvularia lunata,PT12-1 as Diaporthe gulyae,and PT12-5 as Fusarium oxysporum.The pathogenicity assays revealed that all four fungal strains were pathogenic to grape leaves,thereby fulfilling Koch's postulates.Antagonistic experiments indicated that the three selected antagonistic bacteria effectively inhibited the four pathogenic fungi,achieving a maximum inhibition rate of 78.48％.Furthermore,plate experiments utilizing bacterial fermentation broth demonstrated that the fermentation broths of the three antagonistic bacteria significantly inhibited the mycelial growth of the pathogenic fungi,with an inhibition rate exceeding 60％.关键词
葡萄/病原真菌/拮抗细菌/芽孢杆菌/ITS序列分析Key words
grapes/pathogenic fungi/antagonistic bacteria/Bacillus spp./ITS sequence analyses引用本文复制引用
郭絮语,康越,罗春旭,张超,王芳..葡萄病原真菌分离鉴定及拮抗细菌筛选[J].黑龙江农业科学,2025,(7):51-60,10.基金项目
黑龙江省省属本科高校基本科研业务费(145309327). （145309327）
