黑龙江水产2025，Vol.44Issue(4)：403-409,7.
中华绒螯蟹养殖环境中二尖梅奇酵母的时空分布规律研究
Spatiotemporal distribution patterns of Metschnikowia bicuspidata in Chinese mitten crab(Eriocheir sinensis)aquaculture environments
摘要
Abstract
The"milky disease"in Chinese mitten crab(Eriocheir sinensis),caused by the pathogenic yeast Metschnikowia bicuspidata,severely threatens aquaculture productivity.This study systematically monitored spatio-temporal variations of M.bicuspidata concentrations(in CFU/mL for water;CFU/g for sediment)from rice fields and overwintering ponds in Panjin,using selective culture on Rose Bengal agar supplemented with chloramphenicol(50 μg/mL).Results revealed that in disease-affected rice fields,yeast concentrations in water samples peaked significantly in May and September,while sediment samples exhibited elevated levels during February-May and September-November.Unaffected fields maintained lower baseline concentrations,though transient increases were observed in water during October and in sediment during March-April.Overwintering ponds sustained high yeast concentrations throughout November to March,peaking in February,which temporally coincided with milky disease outbreaks.Key factors influencing the distribution included environmental temperature,Eriocheir sinensis biomass,and anthropogenic activities such as harvesting and drainage.Based on these findings,we recommend enhanced environmental disinfection during July-October and post-drainage periods,coupled with optimized water quality management in overwintering ponds,to mitigate disease transmission.These findings suggest that enhanced envi-ronmental management,including disinfection during July-October and post-drainage periods,and optimized water quality control in overwintering ponds,may mitigate disease transmission.关键词
中华绒螯蟹(Eriocheir sinensis)/二尖梅奇酵母(Metschnikowia bicuspidata)/牛奶病/分布规律/养殖环境Key words
Eriocheir sinensis/Metschnikowia bicuspidata/milky disease/spatiotemporal dynamics/aquaculture en-vironments分类
农业科技引用本文复制引用
白永安,秦虎,邢冬飞,梁芳,张冬静,刘广来,李冰玉..中华绒螯蟹养殖环境中二尖梅奇酵母的时空分布规律研究[J].黑龙江水产,2025,44(4):403-409,7.基金项目
国家重点专项项目"海洋农业与淡水渔业科技创新项目:中华绒螯蟹、克氏原螯虾与螺蛳高效制繁种和绿色碳汇养殖示范(项目编号:2022YFD2400705)" （项目编号:2022YFD2400705）
