华南农业大学学报2025，Vol.46Issue(4)：439-449,11.DOI:10.7671/j.issn.1001-411X.202402016
利用分子标记辅助选育不同Wx与ALK基因组合的水稻品系
Molecular marker-assisted breeding of rice strains with different combinations of Wx and ALK genes
摘要
Abstract
[Objective]Wx and ALK are two major genes affecting rice eating and cooking quality,and their allelic combinations have different effects on rice taste quality.The aim of this study was to breed rice strains with different Wx and ALK allelic combinations using molecular marker-assisted selection,and meet people's needs for various tastes and uses.[Method]'Hang Xiangsimiao 3'was used as the donor parent,and'Huahang Tezhan'and'Hang Juxiangsimiao'were used as the backcross parents respectively,to construct BC1F4 populations.Various Wx and ALK combinations were screened with molecular markers.Meanwhile,the rice quality traits of the candidate strains were analyzed.[Result]Three candidate lines were selected from'Huahang Tezhan'backcross progenies,two of which carried WxbALKc and one carried WxaALKc.Fourteen candidate lines were selected from'Hang Juxiangsimiao'backcross progenies,five of which carried WxbALKc and nine carried WxbALKb.Compared with the strains with WxbALKb,the strains with WxbALKc had similar amylose contents,but higher gel consistency and breakdown viscosity,and lower setback viscosity,which made their tastes soft and smooth.One strain with WxaALKc not only had high amylose content(ω)of 32.2%but also high gel consistency,which was suitable for special applications such as rice flour processing.[Conclusion]Total seventeen rice strains with different combinations of Wx and ALK are screened out and bred using molecular markers.These candidate strains have different eating and cooking qualities,and also have multiple uses,providing germplasm resources for breeding of new rice strains which meet people's diversified needs for various tastes and uses in the future.关键词
蜡质基因/糊化温度基因/水稻/稻米品质/分子标记辅助选择Key words
Wx gene/ALK gene/Oryza sativa L./Rice grain quality/Molecular marker-assisted selection分类
农业科技引用本文复制引用
付澳秋,欧旭华,杨雪丽,张思琪,周华,郭涛,杨瑰丽,刘永柱..利用分子标记辅助选育不同Wx与ALK基因组合的水稻品系[J].华南农业大学学报,2025,46(4):439-449,11.基金项目
岭南现代农业科学与技术广东省实验室茂名分中心基金(2022KF003) （2022KF003）
上饶市"揭榜挂帅"项目(2022A002) （2022A002）
崖州湾种子实验室与中国种子集团有限公司联合揭榜挂帅项目(B23YQ1512,B23CQ15CP) （B23YQ1512,B23CQ15CP）
