华南农业大学学报2025，Vol.46Issue(4)：480-487,8.DOI:10.7671/j.issn.1001-411X.202504008
不同杀菌剂对水稻新致病菌菠萝泛菌Pantoea ananatis的防效研究
Study on the efficacy of different fungicides against rice new pathogen Pantoea ananatis
摘要
Abstract
[Objective]To screen highly effective fungicides against rice leaf blight caused by the new pathogen Pantoea ananatis.[Method]Total 16 fungicides were screened via in vitro antibacterial experiments.The effects of three commercial agents on P.ananatis were systematically evaluated by apoptosis,reactive oxygen species(ROS)accumulation and in vivo efficacy.[Result]Both 3 g/L tetramycin AS and 80%(ω)ethylicin EC had good antibacterial effects against P.ananatis,with EC50 of 0.76 and 3.70 μg/mL,respectively.Further mechanism research found that 3 g/L tetramycin AS obviousily induced apoptosis of P.ananatis,increasing the early apoptosis rate to 55.89%,and markedly boosted the level of ROS,causing a marked rise in fluorescence intensity.In the efficacy test of rice bacterial leaf blight caused by P.ananatis,the protective activity of 80%ethylicin EC reached 73.18%,significantly outperforming 3 g/L tetramycin AS(60.71%).Conversely,3 g/L tetramycin AS showed remarkable therapeutic activity at 69.32%.[Conclusion]Tetracymycin can be used to treat the disease effectively by inducing apoptosis and ROS accumulation after the occurrence of the disease,while the outstanding protective activity of ethylicin is more suitable for early prevention.关键词
水稻叶枯病/菠萝泛菌/杀菌剂/治疗活性/保护活性Key words
Rice leaf blight/Pantoea ananatis/Fungicide/Therapeutic activity/Protective activity分类
农业科技引用本文复制引用
陈良业,徐锐涛,蔡昀辰,史瑜,崔紫宁..不同杀菌剂对水稻新致病菌菠萝泛菌Pantoea ananatis的防效研究[J].华南农业大学学报,2025,46(4):480-487,8.基金项目
广东省现代农业产业技术体系创新团队建设项目(2024CXTD21) （2024CXTD21）
