华南农业大学学报2025，Vol.46Issue(4)：488-498,11.DOI:10.7671/j.issn.1001-411X.202501008
四川草地贪夜蛾种群对高效氯氰菊酯的抗药性及代谢途径
Resistance and metabolic pathway of Spodoptera frugiperda population to beta cypermethrin in Sichuan
摘要
Abstract
[Objective]To investigate the resistance and metabolic pathways of beta cypermethrin in fall armyworm(Spodoptera frugiperda)populations in Sichuan.[Method]The resistance levels of fall armyworm to beta cypermethrin in MY,DC,CX,HD and RH populations in Sichuan were determined by drip method.The activities and gene expressions of three kinds of detoxification metabolic enzymes,such as cytochrome P450 enzymes(P450s),carboxylesterases(CarE)and glutathione S-transferase(GST),were determined by photoelectric colorimetric method and RT-qPCR.The Pearson correlation coefficient was used to analyze the correlation of beta cypermethrin resistance with enzyme activity and gene expressions.The differences in beta cypermethrin metabolic products between the MY population treated with LD50 beta cypermethrin(the experimental group)and the untreated MY population(the control group)were analyzed by high performance liquid chromatography-mass spectrometry(HPLC-MS).[Result]The MY population showed the highest resistance ratio(RR)to beta cypermethrin,the RR was 4.02 times and the LD50 was 84.201 μg/g.The activities of CarE,GST and P450s increased with the increase of beta cypermethrin resistance,and the expression levels of CES12,GST epsilon9 and CYP6B50 genes were significantly up-regulated and significantly correlated with beta cypermethrin resistance.The metabolism differential products in the control group and the experimental group were 3-phenoxybenzoic acid(3-PBA),pyrocatechol,capric acid,methyl-2,3-dihydro-3,5-dihydroxy-2-oxo-3-indoleacetic acid.Among them,3-PBA was only detected in the experimental group and not in the control group.It was inferred that beta cypermethrin was metabolized into 3-PBA through the enzyme activity of fall armyworm,3-PBA was catalyzed by monooxygenase,two hydroxyl groups were introduced into benzene ring,and then pyrocatechol was finally generated.[Conclusion]Spodoptera frugiperda may up-regulate the expression of genes associated with detoxification enzymes,leading to increased enzyme activities.This enhanced detoxification capacity accelerates the metabolic degradation of beta cypermethrin,thereby reducing the pesticide's efficacy.关键词
草地贪夜蛾/高效氯氰菊酯/解毒酶/HPLC-MS/代谢途径Key words
Spodoptera frugiperda/Beta cypermethrin/Detoxification enzyme/HPLC-MS/Metabolic pathway分类
农业科技引用本文复制引用
陈慧淋,吴玉童,高节,徐翔,王学贵,杨继芝..四川草地贪夜蛾种群对高效氯氰菊酯的抗药性及代谢途径[J].华南农业大学学报,2025,46(4):488-498,11.基金项目
四川省重点研发计划(2022YFSY0034) （2022YFSY0034）
