河南农业科学2025，Vol.54Issue(7)：12-20,9.DOI:10.15933/j.cnki.1004-3268.2025.07.002
水稻苗期耐旱性全基因组关联分析及候选基因预测
Genome-wide Association Analysis and Candidate Gene Prediction for Rice Drought Tolerance at Seedling Stage
摘要
Abstract
Taking 355 rice germplasms from RDP-Ⅱ as materials,taking the yellow leaf rate after re-watering as the drought tolerance evaluation index,combined with the population genotype data,genome-wide association analysis(GWAS)was carried out,and the candidate genes related to drought tolerance were analyzed,so as to provide excellent germplasm resources and gene resources for the breeding of drought tolerant rice.The results showed that in RDP-Ⅱ population,the yellow leaf rate of tropical japonica rice was the lowest,and that of Osbeck rice was the highest;Seventeen SNP loci related to drought tolerance were identified by GWAS,and distributed on chromosomes 1,2,3,6,7,8,11 and 12 of rice.Candidate genes were predicted in the 10 kb region near the peak SNP,and 33 candidate genes related to drought tolerance were screened,mainly including genes encoding protein kinases,transposon proteins,etc.Based on gene function annotation,8 candidate genes were selected for gene expression analysis in drought tolerant germplasm and drought intolerant germplasm,and OsCTFdt and OsPEX1 genes with significant difference in expression level were identified,which positively and negatively regulated the drought tolerance of rice at seedling stage,respectively.OsCTFdt and OsPEX1 genes had 4 haplotypes respectively,and the yellow leaf rate of the dominant haplotype was significantly lower than that of other haplotypes.关键词
水稻/苗期耐旱性/全基因组关联分析/候选基因预测/单倍型Key words
Rice/Drought tolerance at seedling stage/Genome wide association analysis/Candidate gene prediction/Haplotype分类
农业科技引用本文复制引用
黄世鹏,何虎强,李光照,陈传鑫,钟拓,谢剑波,周湘晚,王丹,肖应辉..水稻苗期耐旱性全基因组关联分析及候选基因预测[J].河南农业科学,2025,54(7):12-20,9.基金项目
湖南省自然科学基金项目(2022JJ30296) （2022JJ30296）
