河南农业科学2025，Vol.54Issue(7)：40-54,15.DOI:10.15933/j.cnki.1004-3268.2025.07.005
油莎豆苗期耐盐碱性综合评价及指标筛选
Comprehensive Evaluation and Index Screening of Salt-Alkali Tolerance during the Seedling Stage of Cyperus esculentus
摘要
Abstract
To clarify the salt-alkali tolerance characteristics of tiger nut(Cyperus esculentus)at the seedling stage and establish precise evaluation indicators and a relevant mathematical model,18 trait indices including germination rate,plant height,root length,root number,plant biomass,plant water content,chlorophyll content,superoxide dismutase(SOD)activity,and soluble protein content were measured for 66 tiger nut germplasm resources under 5 g/kg mixed salt-alkali stress after 15 days of germination.Comprehensive analytical methods,including correlation analysis,principal component analysis(PCA),membership function,grey relational analysis,and stepwise regression analysis,were employed to assess the stress tolerance performance of different materials and establish an evaluation system.The results showed that the variation in salt-alkali tolerance coefficients among different traits ranged from 9.79%to 58.57%,with the coefficient for root dry mass exhibiting the greatest variability and that for relative water content demonstrating the smallest variability.PCA transformed the 18 original indicators into six principal components,which cumulatively accounted for 86.783%of the total variance.Based on the comprehensive evaluation D-value,the 66 germplasm resources were classified into five tolerance levels:extremely strong,strong,moderate,weak,and sensitive.Classification criteria were defined,identifying two germplasm resources with extremely strong tolerance and 12 salt-sensitive germplasm resources.Cultivars Yu Yousha 2 and Yu Yousha 3 were both classified as strongly salt-alkali tolerant materials.Using stepwise regression analysis,a mathematical model for evaluating salt-alkali tolerance at the seedling stage was established.This model demonstrates that by measuring and calculating the salt-alkali tolerance coefficients of total fresh weight,relative water content,root number,total chlorophyll content,plant height,and SOD activity,the D-value can be estimated using the regression equation to assess the salt-alkali tolerance of tiger nut germplasms at the seedling stage.This study provides essential materials,methodological support,and a robust evaluation framework for subsequent research on salt-alkali stress tolerant mechanisms and breeding of tiger nut varieties.关键词
油莎豆/苗期/混合盐碱胁迫/种质资源筛选/综合评价/评价指标Key words
Cyperus esculentus/Seedling stage/Mixed salt-alkaline stress/Selection of germplasm resources/Comprehensive evaluation/Evaluation index分类
农业科技引用本文复制引用
李春鑫,翟允汝,王树峰,陈献功,郑梅,王猛,张戈,王会伟,袁天佑..油莎豆苗期耐盐碱性综合评价及指标筛选[J].河南农业科学,2025,54(7):40-54,15.基金项目
河南省科技攻关项目(232102110188) （232102110188）
河南省中央引导地方科技发展资金项目(Z20231811175) （Z20231811175）
河南省农业科学院新兴学科发展专项(2024XK02) （2024XK02）
河南省农业科学院自主创新项目(2024ZC019) （2024ZC019）
