河南农业科学2025，Vol.54Issue(7)：64-71,8.DOI:10.15933/j.cnki.1004-3268.2025.07.007
白芷种质遗传多样性与群体结构分析
Genetic Diversity and Population Structure of Angelica dahurica Germplasm Resources
摘要
Abstract
To clarify the genetic diversity and population structure characteristics of Angelica dahurica parent materials and improve the utilization efficiency of A.dahurica germplasm resources,28 start codon targeted polymorphism(SCoT)markers were used for PCR amplification of 78 germplasm accessions from 5 A.dahurica populations.Genetic diversity and population structure were analyzed using softwares such as POPGENE 1.32 and GenALEx6.502.The results showed that 28 SCoT markers amplified a total of 192 bands,including 159 polymorphic bands.The average number of amplified bands and polymorphic bands per marker were 7 and 6,respectively.The average values of polymorphic information content(PIC),Shannon's diversity index(I),and Nei's gene diversity index(H)for SCoT markers were 0.800,0.350,and 0.240,respectively,indicating relatively high genetic diversity.For the five A.dahurica populations,the average number of alleles per locus(Na),average effective number of alleles(Ne),average I,and average expected heterozygosity(He)were 1.514,1.293,0.265,and 0.174,respectively,with the Yu A.dahurica population showing the highest values.The genetic differentiation coefficient and gene flow among the five populations were 0.135 and 3.195,respectively.94%of the genetic variation originated within populations,and high gene flow reduced genetic differences between populations.Based on genetic similarity coefficients(GS)among populations,the five A.dahurica populations were clustered into three groups at a GS threshold of 0.96:Yu A.dahurica,Hang A.dahurica,and Chuan A.dahurica populations were grouped together.Based on the GS of 78 A.dahurica germplasms,they were clustered into three major categories at a GS value of 0.765.These categories included 73,3,and 2 A.dahurica germplasms respectively.In conclusion,the five A.dahurica populations exhibit low levels of genetic diversity and close genetic relationships among germplasms.Therefore,efforts should be strengthened to create new A.dahurica germplasm resources.关键词
白芷/种质/SCoT标记/遗传多样性/群体结构/聚类分析Key words
Angelica dahurica/Germplasm/SCoT marker/Genetic diversity/Population structure/Cluster analysis分类
农业科技引用本文复制引用
许兰杰,安素妨,余永亮,董薇,梁慧珍,谭政委,杨青,杨红旗,吴晓慧..白芷种质遗传多样性与群体结构分析[J].河南农业科学,2025,54(7):64-71,8.基金项目
国家现代农业产业技术体系项目(CARS-21) （CARS-21）
河南省重点研发专项(241111310200,231111110800) （241111310200,231111110800）
河南省农科院基本科研业务费项目(2025ZC44,2025ZC45) （2025ZC44,2025ZC45）
中央本级重大增减支项目(2060302) （2060302）
河南省外籍科学家工作室项目(GZS2024025) （GZS2024025）
河南省自主创新项目(2025ZC44) （2025ZC44）
