河南农业科学2025，Vol.54Issue(7)：84-97,14.DOI:10.15933/j.cnki.1004-3268.2025.07.009
外加碳磷对黔中黄壤微生物群落特征及构建过程的影响
Effects of Carbon and Phosphorus Addition on the Characteristics and Construction Process of Microbial Communities in the Yellow Soil of Central Guizhou
摘要
Abstract
To investigate the effects of external carbon and phosphorus on the characteristics and construction process of soil microbial communities,glucose was used as the exogenouse carbon source material,and potassium dihydrogen phosphate was used as the phosphorus source.Four treatments were set up,including control(CK),carbon addition(C),phosphorus addition(P),and carbon phosphorus addition(CP).Using yellow soil from central Guizhou as the experimental soil,a pot experiment with oilseed rape was conducted to investigate the effects of carbon and phosphorus addition on soil physicochemical properties,microbial community structure,and community assembly.The results showed that the total nitrogen content of the C,P,and CP treatments increased significantly by 7.82%,6.17%,and 6.17%,respectively,compared to the CK treatment,with no significant difference among the three treatments.The content of soil alkali hydrolyzable nitrogen in C,P and CP treatments increased,but there was no significant difference compared with the control.The soil nitrate nitrogen contents in C and P treatments were significantly higher than that in CK and CP treatments.The soil pH value and ammonium nitrogen content in CP treatment were significantly higher than those in other treatments.The oilseed rape yields of the treatments were in the order of CP>C>P>CK.The relative abundance of Proteobacteria and Actinobacteria decreased,while the relative abundance of Chloroflexi and Gemmatimonadetes increased.The addition of carbon and phosphorus promoted the growth of Ascomycota.The addition of carbon and phosphorus had little effect on the α diversity of bacterial and fungal communities.The Mantel test showed that indicators such as soil ammonium nitrogen,organic matter,and available potassium content had a significant impact on bacterial community beta diversity at the P<0.001 level;The indicators of soil ammonia nitrogen and total phosphorus content had a significant impact on the β diversity of fungal communities at the P<0.01 level.The order of smoothness of bacterial network connectivity was P>C>CP>CK,while the order of smoothness of fungal network connectivity was C>P>CK>CP.The construction process of bacterial and fungal communities in each treatment is dominated by stochastic processes.The content of ammonium nitrogen was significantly correlated with bacterial and fungal βNTI values at the P<0.001 level,while pH value and soil organic matter were significantly correlated with bacterial βNTI values(P<0.05 and above)and fungal βNTI values at the P<0.001 level.In summary,the addition of carbon and phosphorus has an impact on soil physical and chemical properties(such as ammonium nitrogen),thereby altering microbial community β diversity and community construction processes.关键词
黄壤/微生物群落/群落构建/碳磷添加/响应Key words
Yellow soil/Microbial community/Community assembly/Carbon and phosphorus addition/Response分类
农业科技引用本文复制引用
秦仕华,从春蕾,闫茹,刘超,宋晓慧,乔志伟..外加碳磷对黔中黄壤微生物群落特征及构建过程的影响[J].河南农业科学,2025,54(7):84-97,14.基金项目
贵州省科技计划项目(黔科合基础[2024]青年286) （黔科合基础[2024]青年286）
贵州省教育厅高等学校科学研究青年项目(黔教技[2022]331) （黔教技[2022]331）
贵州省教育厅水华治理与水生态修复科技创新团队项目(2023085) （2023085）
