中医药导报2025，Vol.31Issue(6)：15-20,6.DOI:10.13862/j.cn43-1446/r.2025.06.003
摩腹对功能性便秘大鼠肠道菌群的影响
The Effect of Abdominal Friction on the Intestinal Microbiota of Rats with Functional Constipation
摘要
Abstract
Objective:To investigate the therapeutic effect and intestinal microbiota of abdominal friction on fluperamide-induced functional constipation in rats.Methods:A total of 40 SD rats were randomly divided into normal group(group N),model group(group M),lactulose group(group L)and Tuina group(group TN),with 10 rats in each group.On the lst to 14th days,group M,group L and group TN were gavaged with a suspension of loperamide hydrochloride capsule powder(3 mg/kg),and group N was gavaged with the same volume of normal saline.On the 15th to 28th days,group N and group M were given normal saline(10 mL/kg),and group L was given lactulose(2.57 ml/kg),once a day.Group TN was given abdominal friction intervention,once a day.After the end of treatment,the fecal moisture content and intestinal propulsion rate of rats in each group were detected,and the collected fresh feces of rats were tested for 16SrDNA to analyze the changes of intestinal flora in the samples.Results:Compared with the group N,the fecal water content decreased significantly in group M(P＜0.05),and the intestinal propulsion rate decreased significantly in group M(P＜0.05).There was disruption of gut microbiota in the group M.The abundance of Firmicuts and Lactobacillaceae decreased in group M,while the abundance of Bacteroidetes,Muribaculaceae,Lachnospiraceae and Prevotel-laceae increased in group M.Compared with the group M,the fecal water content increased significantly in group L and group TN(P＜0.05),and the intestinal propulsion rate increased significantly in group L and group TN(P＜0.05).The abundances of Bacteroidetes,Muribaculacea and Prevotellaceae decreased in group L and group TN,while the abundances of Firmicuts,Lachnospiraceae and Oscillospiraceae increased group L and group TN.LEfSe analysis showed that c-clostridia,p-firmicutes,o-Lachnospirales,f-Lachnospiraceae and o-Oscillospirales were the most abundant differential species in the TN group.Conclusion:By adjusting the structure of the intestinal flora of rats,abdominal friction can improve the constipation state of rats.关键词
功能性便秘/摩腹/肠道菌群/16SrDNA/大鼠Key words
functional constipation/abdominal friction/intestinal flora/16SrDNA/rat分类
医药卫生引用本文复制引用
何丹丹,张贤,刘小峰,邵家豪,廖翼涛,陈莉,杭晓娟,吴昔钧..摩腹对功能性便秘大鼠肠道菌群的影响[J].中医药导报,2025,31(6):15-20,6.基金项目
国家中医药管理局国家中医优势专科建设项目(国中医药政函[2024]90号) （国中医药政函[2024]90号）
无锡市卫生健康委妇幼健康适宜技术推广项目(FYTG202307) （FYTG202307）
江苏省自然科学基金项目(BK20231147) （BK20231147）
无锡市卫生健康委科技成果与适宜技术推广项目(T202338) （T202338）
