摘要
Abstract
Objective:To explore the effect and mechanism of Bushen Huoxue formula on improving renal fibrosis in lupus nephritis rats,and to analyze the clinical therapeutic value of Bushen Huoxue formula on lupus nephritis.Methods:Totally 40 SD rats were selected to create lupus nephritis rat model by intraperitoneal injection of norphytane.The rats were divided into 4 groups,including blank group,model group,prednisone group and Bushen Huoxue formula group.After 28 days of treatment in prednisone group and Bushen Huoxue formula group,the expression levels of blood urea nitrogen(BUN)and serum creatinine(S-Cr)in serum renal function indexes and the contents of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)were detected by enzyme-linked immunosorbent assay(ELISA).Hematoxylin-eosin(HE)and Masson staining were used to observe the pathology of renal tissues in each group.The protein expression level was detected by Western blotting.Result:Compared with the blank group,the serum renal function indexes(BUN and S-Cr)levels,and the pro-inflammatory factor(TNF-α and IL-6)levels were increased in the model group.The degree of renal fibrosis was aggravated,and the fibrosis area was increased in model group.The expression levels of TGF-β1,type Ⅰ and type Ⅲ collagen,the key molecular proteins in the fibrosis process,were increased in model group.Compared with model group,the levels of BUN and S-Cr in serum renal function indexes,and the levels of pro-inflammatory factors TNF-α and IL-6 were decreased in Bushen Huoxue formula group and prednisone group.The degree of renal fibrosis was alleviated Bushen Huoxue formula group and prednisone group.The expression levels of TGF-β1,type Ⅰ and type Ⅲ collagen,the key molecular proteins in the fibro-sis process,were decreased in Bushen Huoxue formula group and prednisone group.p-ERK1/2,a key protein in the ERK pathway,was also reducedin Bushen Huoxue formula group and prednisone group.Conclusion:Bushen Huoxue formula may improve renal fibrosis in lupus rat nephritis by inhibiting p-ERK1/2 pathway.关键词
系统性红斑狼疮/狼疮肾炎/肾纤维化/补肾活血方/pERK1/2通路/大鼠Key words
systemic lupus erythematosus/lupus nephritis/renal fibrosis/Bushen Huoxue formula/p-ERK1/2 signaling pathway/rat分类
医药卫生
