中医药导报2025，Vol.31Issue(6)：26-30,5.DOI:10.13862/j.cn43-1446/r.2025.06.005
基于脂质过氧化途径探讨黄芪甲苷对高糖诱导人足细胞铁死亡的保护作用
To Investigate the Protective Effect of Astragaloside Ⅳ on High Glucose-Induced Ferroptosis in Human Podocytes Based on Lipid Peroxidation Pathway
摘要
Abstract
Objective:To investigate the protective effect of astragaloside Ⅳ on high glucose-induced ferroptosis in human podocytes(HPC)by reducing the occurrence of lipid peroxidation.Methods:The cells were divided into control group(5.5 mmol/L D-glucose),model group(30.0 mmol/L D-glucose),low dose group(30.0 mmol/L D-glucose+10.0 μmol/L AS-Ⅳ)and high dose group(30.0 mmol/L D-glucose+40.0 μmol/L AS-Ⅳ).The cell viability was calculated by CCK-8.The protein levels of cleaved Caspase-3,PTGS2 and GPX4 were detected by Western blotting.The apoptosis was observed by Hoechst staining,and the levels of MDA,SOD,CAT and GSH-Px were detected.Results:Compared with the model group,the cell survival rate increased in low dose group and high dose group(P<0.05),and the expression level of cleaved Caspase-3 decreased in high dose group(P<0.05).Hoechst staining showed that the apoptosis rate decreased in high dose group(P<0.01).The levels of MDA decreased in low dose group and high dose group(P<0.01),and the levels of SOD increased in low dose group and high dose group(P<0.01).The levels of CAT and GSH-px in increased in the low dose group and high dose group(P<0.05 or P<0.01).The expression level of PTGS2 decreased in low dose group and high dose group(P<0.01),while the expression level of GPX4 protein increased in high dose group(P<0.01).Conclusion:Astragaloside Ⅳ may reduce the ferroptosis of glomerular podocytes induced by high glucose according to inhibiting the occurrence of lipid peroxidation.关键词
足细胞/糖尿病肾脏病/铁死亡/黄芪甲苷/脂质过氧化Key words
podocyte/diabetes kidney disease/ferroptosis/astragaloside Ⅳ/lipid peroxidation分类
医药卫生引用本文复制引用
孙亚利,王彬蒙,丁新月,邢丽娜..基于脂质过氧化途径探讨黄芪甲苷对高糖诱导人足细胞铁死亡的保护作用[J].中医药导报,2025,31(6):26-30,5.基金项目
上海市普陀区卫生健康系统科技创新项目(ptkwws202203) （ptkwws202203）
